In vitro culture of Keratinocytes from human umbilical cord blood mesenchymal stem cells : the Saigonese culture
There have been many attempts to acquire and culture human keratinocytes for clinical purposes including from keratotome slices in media with fetal calf serum (FCS) or pituitary extract (PE), from skin specimens in media with feeder layers, from suction blister epidermal roofs' in serum-free culture and from human umbilical cord blood (hUCB) mesenchymal stem cells (MSCs) in media with skin feeder layers. Conversely this study was designed to investigate whether keratinocytes could be obtained directly from hUCB MSCs in vitro. It is widely established that mesenchymal stem cells from human umbilical cord blood have multipotent capacity and the ability to differentiate into disparate cell lineages hUCB MSCs were directly induced to differentiate into keratinocytes by using a specific medium composed of primary culture medium (PCM) and serum free medium (SFM) in a ratio 1:9 for a period of 7 days and tested by immunostain p63 and K1-K10. Cells thus cultured were positive in both tests, confirming the possibility to directly obtain keratinocytes from MSCs hUCB in vitro.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2011 |
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| Erschienen: |
2011 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Cell and tissue banking - 12(2011), 2 vom: 25. Mai, Seite 125-33 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Tran, Cong Toai [VerfasserIn] |
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| Anmerkungen: |
Date Completed 16.08.2011 Date Revised 01.12.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s10561-010-9174-8 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM196942373 |
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| 500 | |a Date Revised 01.12.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a There have been many attempts to acquire and culture human keratinocytes for clinical purposes including from keratotome slices in media with fetal calf serum (FCS) or pituitary extract (PE), from skin specimens in media with feeder layers, from suction blister epidermal roofs' in serum-free culture and from human umbilical cord blood (hUCB) mesenchymal stem cells (MSCs) in media with skin feeder layers. Conversely this study was designed to investigate whether keratinocytes could be obtained directly from hUCB MSCs in vitro. It is widely established that mesenchymal stem cells from human umbilical cord blood have multipotent capacity and the ability to differentiate into disparate cell lineages hUCB MSCs were directly induced to differentiate into keratinocytes by using a specific medium composed of primary culture medium (PCM) and serum free medium (SFM) in a ratio 1:9 for a period of 7 days and tested by immunostain p63 and K1-K10. Cells thus cultured were positive in both tests, confirming the possibility to directly obtain keratinocytes from MSCs hUCB in vitro | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Huynh, Duy Thao |e verfasserin |4 aut | |
| 700 | 1 | |a Gargiulo, Ciro |e verfasserin |4 aut | |
| 700 | 1 | |a Nguyen, Phuong Thao |e verfasserin |4 aut | |
| 700 | 1 | |a Tran, Thi Thanh Thuy |e verfasserin |4 aut | |
| 700 | 1 | |a Huynh, Minh Tuan |e verfasserin |4 aut | |
| 700 | 1 | |a Nguyen, Thanh Tung |e verfasserin |4 aut | |
| 700 | 1 | |a Filgueira, Luis |e verfasserin |4 aut | |
| 700 | 1 | |a Strong, D Micheal |e verfasserin |4 aut | |
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