Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose
Copyright 2010 Elsevier B.V. All rights reserved..
In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 microm diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 microM). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the literature; furthermore, the sensor was able to show that insulin increased the intracellular glucose concentration. This nanoelectrode device demonstrates a simple technique to measure intracellular glucose concentration.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2010 |
|---|---|
| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
|---|---|
| Enthalten in: |
Biosensors & bioelectronics - 25(2010), 10 vom: 15. Juni, Seite 2205-11 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Asif, Muhammad H [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Completed 19.08.2010 Date Revised 21.11.2013 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.bios.2010.02.025 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM196509777 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM196509777 | ||
| 003 | DE-627 | ||
| 005 | 20231223204806.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231223s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.bios.2010.02.025 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0655.xml |
| 035 | |a (DE-627)NLM196509777 | ||
| 035 | |a (NLM)20303253 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Asif, Muhammad H |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose |
| 264 | 1 | |c 2010 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 19.08.2010 | ||
| 500 | |a Date Revised 21.11.2013 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright 2010 Elsevier B.V. All rights reserved. | ||
| 520 | |a In this article, we report a functionalised ZnO-nanorod-based selective electrochemical sensor for intracellular glucose. To adjust the sensor for intracellular glucose measurements, we grew hexagonal ZnO nanorods on the tip of a silver-covered borosilicate glass capillary (0.7 microm diameter) and coated them with the enzyme glucose oxidase. The enzyme-coated ZnO nanorods exhibited a glucose-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode. The potential difference was linear over the concentration range of interest (0.5-1000 microM). The measured glucose concentration in human adipocytes or frog oocytes using our ZnO-nanorod sensor was consistent with values of glucose concentration reported in the literature; furthermore, the sensor was able to show that insulin increased the intracellular glucose concentration. This nanoelectrode device demonstrates a simple technique to measure intracellular glucose concentration | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Glucose |2 NLM | |
| 650 | 7 | |a IY9XDZ35W2 |2 NLM | |
| 650 | 7 | |a Zinc Oxide |2 NLM | |
| 650 | 7 | |a SOI2LOH54Z |2 NLM | |
| 700 | 1 | |a Ali, Syed M Usman |e verfasserin |4 aut | |
| 700 | 1 | |a Nur, Omer |e verfasserin |4 aut | |
| 700 | 1 | |a Willander, Magnus |e verfasserin |4 aut | |
| 700 | 1 | |a Brännmark, Cecilia |e verfasserin |4 aut | |
| 700 | 1 | |a Strålfors, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Englund, Ulrika H |e verfasserin |4 aut | |
| 700 | 1 | |a Elinder, Fredrik |e verfasserin |4 aut | |
| 700 | 1 | |a Danielsson, Bengt |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biosensors & bioelectronics |d 1996 |g 25(2010), 10 vom: 15. Juni, Seite 2205-11 |w (DE-627)NLM012733431 |x 1873-4235 |7 nnns |
| 773 | 1 | 8 | |g volume:25 |g year:2010 |g number:10 |g day:15 |g month:06 |g pages:2205-11 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.bios.2010.02.025 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 25 |j 2010 |e 10 |b 15 |c 06 |h 2205-11 | ||