Epithelial-mesenchymal transition induced by hepatitis C virus core protein in cholangiocarcinoma
BACKGROUND: Cholangiocarcinoma (CC) is associated with chronic hepatitis C virus (HCV) infection. We investigated the effect of hepatitis C virus core protein (HCVc) on epithelial-mesenchymal transition (EMT) in CC and tried to identify its target trigger.
METHODS: First, we examined expression of HCVc and epithelial and mesenchymal markers in CC tissues. Then we transient-transfected HCVc gene into a CC cell line and examined expression of lysyl oxidase-like 2 (LOXL2) and epithelial and mesenchymal markers by quantitative real-time polymerase chain reaction (PCR) and Western blotting. Finally, LOXL2 gene silencing was shown in QBC939/HCVc cells by RNA interference (RNAi), and we further examined expression of epithelial and mesenchymal markers by quantitative real-time PCR and Western blotting.
RESULTS: Through immunohistochemical staining, the present study showed that HCVc is significantly associated with CC invasion and metastasis. In vitro study showed that HCVc expression induces EMT in CC cell line QBC939, and a mechanism through LOXL2 pathway is suggested. Expression of HCVc was significantly correlated with greater migratory and invasive potential of CC cells.
CONCLUSIONS: These observations indicate that HCVc plays a critical role in promoting invasion and metastasis of CC cells.
| Errataetall: |
CommentIn: Ann Surg Oncol. 2011 Mar;18(3):896; author reply 897. - PMID 20645009 |
|---|---|
| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2010 |
|---|---|
| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
|---|---|
| Enthalten in: |
Annals of surgical oncology - 17(2010), 7 vom: 07. Juli, Seite 1937-44 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Li, Tianyu [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 28.09.2010 Date Revised 29.03.2011 published: Print-Electronic CommentIn: Ann Surg Oncol. 2011 Mar;18(3):896; author reply 897. - PMID 20645009 Citation Status MEDLINE |
|---|
| doi: |
10.1245/s10434-010-0925-3 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM195160193 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM195160193 | ||
| 003 | DE-627 | ||
| 005 | 20231223202211.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231223s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1245/s10434-010-0925-3 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0651.xml |
| 035 | |a (DE-627)NLM195160193 | ||
| 035 | |a (NLM)20162464 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Li, Tianyu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Epithelial-mesenchymal transition induced by hepatitis C virus core protein in cholangiocarcinoma |
| 264 | 1 | |c 2010 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 28.09.2010 | ||
| 500 | |a Date Revised 29.03.2011 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a CommentIn: Ann Surg Oncol. 2011 Mar;18(3):896; author reply 897. - PMID 20645009 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Cholangiocarcinoma (CC) is associated with chronic hepatitis C virus (HCV) infection. We investigated the effect of hepatitis C virus core protein (HCVc) on epithelial-mesenchymal transition (EMT) in CC and tried to identify its target trigger | ||
| 520 | |a METHODS: First, we examined expression of HCVc and epithelial and mesenchymal markers in CC tissues. Then we transient-transfected HCVc gene into a CC cell line and examined expression of lysyl oxidase-like 2 (LOXL2) and epithelial and mesenchymal markers by quantitative real-time polymerase chain reaction (PCR) and Western blotting. Finally, LOXL2 gene silencing was shown in QBC939/HCVc cells by RNA interference (RNAi), and we further examined expression of epithelial and mesenchymal markers by quantitative real-time PCR and Western blotting | ||
| 520 | |a RESULTS: Through immunohistochemical staining, the present study showed that HCVc is significantly associated with CC invasion and metastasis. In vitro study showed that HCVc expression induces EMT in CC cell line QBC939, and a mechanism through LOXL2 pathway is suggested. Expression of HCVc was significantly correlated with greater migratory and invasive potential of CC cells | ||
| 520 | |a CONCLUSIONS: These observations indicate that HCVc plays a critical role in promoting invasion and metastasis of CC cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Hepatitis C Antigens |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a RNA, Small Interfering |2 NLM | |
| 650 | 7 | |a Viral Core Proteins |2 NLM | |
| 650 | 7 | |a nucleocapsid protein, Hepatitis C virus |2 NLM | |
| 650 | 7 | |a Amino Acid Oxidoreductases |2 NLM | |
| 650 | 7 | |a EC 1.4.- |2 NLM | |
| 650 | 7 | |a LOXL2 protein, human |2 NLM | |
| 650 | 7 | |a EC 1.4.3.- |2 NLM | |
| 700 | 1 | |a Li, Dajiang |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Long |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Hongye |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Zhanfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Zipei |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Weiwei |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Ying Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Lijin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Shuguang |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Annals of surgical oncology |d 1995 |g 17(2010), 7 vom: 07. Juli, Seite 1937-44 |w (DE-627)NLM074734997 |x 1534-4681 |7 nnns |
| 773 | 1 | 8 | |g volume:17 |g year:2010 |g number:7 |g day:07 |g month:07 |g pages:1937-44 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1245/s10434-010-0925-3 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 17 |j 2010 |e 7 |b 07 |c 07 |h 1937-44 | ||