Identification of novel L-amino acid alpha-ligases through Hidden Markov Model-based profile analysis
L-Amino acid alpha-ligase (Lal), catalyzing the formation of alpha-dipeptides from unprotected L-amino acids in an ATP-dependent manner, is used in cost-effective fermentative production of dipeptides. We searched for novel Lals by in silico screening using Hidden Markov Model-based profile analysis, and identified five novel Lals that showed low similarity and different substrate specificity from known Lals.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2010 |
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| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:74 |
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| Enthalten in: |
Bioscience, biotechnology, and biochemistry - 74(2010), 2 vom: 05., Seite 415-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Senoo, Akihiro [VerfasserIn] |
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| Themen: |
Amino Acids |
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| Anmerkungen: |
Date Completed 20.05.2010 Date Revised 11.09.2019 published: Print-Electronic Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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NLM19494459X |
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| 520 | |a L-Amino acid alpha-ligase (Lal), catalyzing the formation of alpha-dipeptides from unprotected L-amino acids in an ATP-dependent manner, is used in cost-effective fermentative production of dipeptides. We searched for novel Lals by in silico screening using Hidden Markov Model-based profile analysis, and identified five novel Lals that showed low similarity and different substrate specificity from known Lals | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Amino Acids |2 NLM | |
| 650 | 7 | |a Dipeptides |2 NLM | |
| 650 | 7 | |a Oligopeptides |2 NLM | |
| 650 | 7 | |a Recombinant Proteins |2 NLM | |
| 650 | 7 | |a Ligases |2 NLM | |
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| 700 | 1 | |a Tabata, Kazuhiko |e verfasserin |4 aut | |
| 700 | 1 | |a Yonetani, Yoshiyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Yagasaki, Makoto |e verfasserin |4 aut | |
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