Assessing the insecticide resistance of an Aedes aegypti strain in El Salvador
OBJECTIVES: To assess the level of insecticide susceptibility of a certain Aedes aegypti strain found in El Salvador and to explain the mechanisms for its resistance to temephos.
METHODS: An A. aegypti strain from the municipality of Soyapango, Department of San Salvador, El Salvador, was studied. Bioassays were used to determine the susceptibility of the larvae to the organophosphate insecticide temephos and to three pyrethroids (deltamethrin, lambda-cyhalothrin, and cypermethrin); and of adults to an organophosphate insecticide (chlorpyrifos). The resistance factor (RF50) with determined with respect to a reference susceptible strain (Rockefeller). The mechanism of temephos resistance was determined through the use of synergistic substances, biochemical assays for enzymatic activity, and polyacrylamide gel zymograms.
RESULTS: The larvae of the strain studied proved highly resistant to temephos (RF50 = 24.16). Of the enzyme samples analyzed, only the esterase A4 was linked to the mechanism of temephos resistance. The adult mosquitoes were susceptible to lambda-cyhalothrin and chlorpyrifos; and resistance to deltamethrin and cypermethrin fell into the category needing further verification.
CONCLUSIONS: Temephos resistance could reduce the efficiency of chemical control of the A. aegypti mosquito in El Salvador study area. Chlorpyrifos, lambda-cyhalothrin, and cypermethrin are good alternative insecticides for use in new efforts to control this vector.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2009 |
|---|---|
| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
|---|---|
| Enthalten in: |
Revista panamericana de salud publica = Pan American journal of public health - 26(2009), 3 vom: 20. Sept., Seite 229-34 |
| Sprache: |
Spanisch |
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| Weiterer Titel: |
Evaluación de la resistencia a insecticidas de una cepa de Aedes aegypti de El Salvador |
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| Beteiligte Personen: |
Lazcano, Juan A Bisset [VerfasserIn] |
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| Themen: |
English Abstract |
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| Anmerkungen: |
Date Completed 03.03.2010 Date Revised 17.05.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM194213099 |
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| 100 | 1 | |a Lazcano, Juan A Bisset |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Assessing the insecticide resistance of an Aedes aegypti strain in El Salvador |
| 246 | 3 | 3 | |a Evaluación de la resistencia a insecticidas de una cepa de Aedes aegypti de El Salvador |
| 264 | 1 | |c 2009 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
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| 500 | |a Date Completed 03.03.2010 | ||
| 500 | |a Date Revised 17.05.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVES: To assess the level of insecticide susceptibility of a certain Aedes aegypti strain found in El Salvador and to explain the mechanisms for its resistance to temephos | ||
| 520 | |a METHODS: An A. aegypti strain from the municipality of Soyapango, Department of San Salvador, El Salvador, was studied. Bioassays were used to determine the susceptibility of the larvae to the organophosphate insecticide temephos and to three pyrethroids (deltamethrin, lambda-cyhalothrin, and cypermethrin); and of adults to an organophosphate insecticide (chlorpyrifos). The resistance factor (RF50) with determined with respect to a reference susceptible strain (Rockefeller). The mechanism of temephos resistance was determined through the use of synergistic substances, biochemical assays for enzymatic activity, and polyacrylamide gel zymograms | ||
| 520 | |a RESULTS: The larvae of the strain studied proved highly resistant to temephos (RF50 = 24.16). Of the enzyme samples analyzed, only the esterase A4 was linked to the mechanism of temephos resistance. The adult mosquitoes were susceptible to lambda-cyhalothrin and chlorpyrifos; and resistance to deltamethrin and cypermethrin fell into the category needing further verification | ||
| 520 | |a CONCLUSIONS: Temephos resistance could reduce the efficiency of chemical control of the A. aegypti mosquito in El Salvador study area. Chlorpyrifos, lambda-cyhalothrin, and cypermethrin are good alternative insecticides for use in new efforts to control this vector | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 700 | 1 | |a Rodríguez, María M |e verfasserin |4 aut | |
| 700 | 1 | |a San Martín, José L |e verfasserin |4 aut | |
| 700 | 1 | |a Romero, José E |e verfasserin |4 aut | |
| 700 | 1 | |a Montoya, Romeo |e verfasserin |4 aut | |
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