Occurrence of mixed populations of influenza A viruses that can be maintained through transmission in a single host and potential for reassortment
Reassortment, which is the rearrangement of viral gene segments in a host cell infected with two different viruses, is an important mechanism for the evolution of influenza viruses. Mixed infections with multiple virus types could lead to reassortment. To better understand the occurrence of quasispecies in a single host, we investigated mixed infections in individual isolates of seasonal influenza A viruses using amantadine sensitivity as a marker. We cultured viruses with amantadine and performed sequencing, restriction fragment length polymorphism analysis, cloning, and quantitative PCR to detect mixed populations. Culturing with amantadine showed evidence of a high number of mixed populations, while the other assays could hardly detect mixed populations. The existence of quasispecies in each isolate was common. However, the proportion of these, which can be less than 1%, is too low to be detected by conventional methods. Such mixed populations in which reassortment occurs may have a significant role in the evolution of viruses.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2010 |
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| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:48 |
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| Enthalten in: |
Journal of clinical microbiology - 48(2010), 2 vom: 15. Feb., Seite 369-74 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Furuse, Yuki [VerfasserIn] |
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| Links: |
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| Themen: |
Amantadine |
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| Anmerkungen: |
Date Completed 29.03.2010 Date Revised 31.03.2022 published: Print-Electronic GENBANK: AB520865, AB520866, AB520867, AB520868, AB520869, AB520870, AB520871, AB520872, AB520873, AB520874, AB520875, AB520876, AB520877, AB520878, AB520879, AB520880, AB520881, AB520882, AB520883, AB520884, AB520885, AB520886, AB520887, AB520888, AB520889, AB520890, AB520891, AB520892, AB520893, AB520894, AB520895, AB520896, AB520897, AB520898, AB520899, AB520900, AB520901, AB520902, AB520903, AB520904, AB520905, AB520906, AB520907, AB520908, AB520909, AB520910, AB520911, AB520912, AB520913, AB520914, AB520915, AB520916, AB520917, AB520918, AB520919, AB520920, AB520921, AB520922, AB520923, AB520924, AB520925, AB520926, AB520927, AB520928, AB520929, AB520930, AB520931, AB520932 Citation Status MEDLINE |
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| doi: |
10.1128/JCM.01795-09 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM193074710 |
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| 245 | 1 | 0 | |a Occurrence of mixed populations of influenza A viruses that can be maintained through transmission in a single host and potential for reassortment |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Reassortment, which is the rearrangement of viral gene segments in a host cell infected with two different viruses, is an important mechanism for the evolution of influenza viruses. Mixed infections with multiple virus types could lead to reassortment. To better understand the occurrence of quasispecies in a single host, we investigated mixed infections in individual isolates of seasonal influenza A viruses using amantadine sensitivity as a marker. We cultured viruses with amantadine and performed sequencing, restriction fragment length polymorphism analysis, cloning, and quantitative PCR to detect mixed populations. Culturing with amantadine showed evidence of a high number of mixed populations, while the other assays could hardly detect mixed populations. The existence of quasispecies in each isolate was common. However, the proportion of these, which can be less than 1%, is too low to be detected by conventional methods. Such mixed populations in which reassortment occurs may have a significant role in the evolution of viruses | ||
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| 700 | 1 | |a Nukiwa, Nao |e verfasserin |4 aut | |
| 700 | 1 | |a Shimizu, Midori |e verfasserin |4 aut | |
| 700 | 1 | |a Sawayama, Rumi |e verfasserin |4 aut | |
| 700 | 1 | |a Fuji, Naoko |e verfasserin |4 aut | |
| 700 | 1 | |a Oshitani, Hitoshi |e verfasserin |4 aut | |
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