Glyceraldehyde-3-phosphate, a glycolytic intermediate, plays a key role in controlling cell fate via inhibition of caspase activity
Glyceraldehyde-3-phosphate is a key intermediate in several central metabolic pathways of all organisms. Aldolase and glyceraldehyde-3-phosphate dehydrogenase are involved in the production or elimination of glyceraldehyde-3-phosphate during glycolysis or gluconeogenesis, and are differentially expressed under various physiological conditions, including cancer, hypoxia, and apoptosis. In this study, we examine the effects of glyceraldehyde-3-phosphate on cell survival and apoptosis. Overexpression of aldolase protected cells against apoptosis, and addition of glyceraldehyde-3-phosphate to cells delayed apoptosis. Additionally, delayed apoptotic phenomena were observed when glyceraldehyde-3-phosphate was added to a cell-free system, in which artificial apoptotic process was induced by adding dATP and cytochrome c. Surprisingly, glyceraldehyde-3-phosphate directly suppressed caspase-3 activity in a reversible noncompetitive mode, preventing caspase-dependent proteolysis. Based on these results, we suggest that glyceraldehyde-3-phosphate, a key molecule in several central metabolic pathways, functions as a molecule switch between cell survival and apoptosis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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| Enthalten in: |
Molecules and cells - 28(2009), 6 vom: 31. Dez., Seite 559-63 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jang, Mi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 11.06.2010 Date Revised 04.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s10059-009-0151-7 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM193049201 |
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| 520 | |a Glyceraldehyde-3-phosphate is a key intermediate in several central metabolic pathways of all organisms. Aldolase and glyceraldehyde-3-phosphate dehydrogenase are involved in the production or elimination of glyceraldehyde-3-phosphate during glycolysis or gluconeogenesis, and are differentially expressed under various physiological conditions, including cancer, hypoxia, and apoptosis. In this study, we examine the effects of glyceraldehyde-3-phosphate on cell survival and apoptosis. Overexpression of aldolase protected cells against apoptosis, and addition of glyceraldehyde-3-phosphate to cells delayed apoptosis. Additionally, delayed apoptotic phenomena were observed when glyceraldehyde-3-phosphate was added to a cell-free system, in which artificial apoptotic process was induced by adding dATP and cytochrome c. Surprisingly, glyceraldehyde-3-phosphate directly suppressed caspase-3 activity in a reversible noncompetitive mode, preventing caspase-dependent proteolysis. Based on these results, we suggest that glyceraldehyde-3-phosphate, a key molecule in several central metabolic pathways, functions as a molecule switch between cell survival and apoptosis | ||
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| 700 | 1 | |a Kang, Hyo Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Sun Young |e verfasserin |4 aut | |
| 700 | 1 | |a Chung, Sang J |e verfasserin |4 aut | |
| 700 | 1 | |a Kang, Sunghyun |e verfasserin |4 aut | |
| 700 | 1 | |a Chi, Seung Wook |e verfasserin |4 aut | |
| 700 | 1 | |a Cho, Sayeon |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Sang Chul |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Chong-Kil |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Byoung Chul |e verfasserin |4 aut | |
| 700 | 1 | |a Bae, Kwang-Hee |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Sung Goo |e verfasserin |4 aut | |
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