The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport
The retromer is a protein complex that mediates retrograde transport of transmembrane cargoes from endosomes to the trans-Golgi network (TGN). It is comprised of a cargo-selection subcomplex of Vps26, Vps29 and Vps35 and a membrane-binding coat subcomplex of sorting nexins (SNXs). Previous studies identified SNX1/2 as one of the components of the SNX subcomplex, and SNX5/6 as candidates for the second SNX. How the retromer-associated cargoes are recognized and transported by molecular motors are largely unknown. In this study, we found that one of SNX1/2's dimerization partners, SNX6, interacts with the p150(Glued) subunit of the dynein/dynactin motor complex. We present evidence that SNX6 is a component of the retromer, and that recruitment of the motor complex to the membrane-associated retromer requires the SNX6-p150(Glued) interaction. Disruption of the SNX6-p150(Glued) interaction causes failure in formation and detachment of the tubulovesicular sorting structures from endosomes and results in block of CI-MPR retrieval from endosomes to the TGN. These observations indicate that in addition to SNX1/2, SNX6 in association with the dynein/dynactin complex drives the formation and movement of tubular retrograde intermediates.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2009 |
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Erschienen: |
2009 |
Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
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Enthalten in: |
Cell research - 19(2009), 12 vom: 25. Dez., Seite 1334-49 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Hong, Zhi [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 26.02.2010 Date Revised 10.03.2022 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1038/cr.2009.130 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM193036460 |
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245 | 1 | 4 | |a The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport |
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520 | |a The retromer is a protein complex that mediates retrograde transport of transmembrane cargoes from endosomes to the trans-Golgi network (TGN). It is comprised of a cargo-selection subcomplex of Vps26, Vps29 and Vps35 and a membrane-binding coat subcomplex of sorting nexins (SNXs). Previous studies identified SNX1/2 as one of the components of the SNX subcomplex, and SNX5/6 as candidates for the second SNX. How the retromer-associated cargoes are recognized and transported by molecular motors are largely unknown. In this study, we found that one of SNX1/2's dimerization partners, SNX6, interacts with the p150(Glued) subunit of the dynein/dynactin motor complex. We present evidence that SNX6 is a component of the retromer, and that recruitment of the motor complex to the membrane-associated retromer requires the SNX6-p150(Glued) interaction. Disruption of the SNX6-p150(Glued) interaction causes failure in formation and detachment of the tubulovesicular sorting structures from endosomes and results in block of CI-MPR retrieval from endosomes to the TGN. These observations indicate that in addition to SNX1/2, SNX6 in association with the dynein/dynactin complex drives the formation and movement of tubular retrograde intermediates | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a DCTN1 protein, human |2 NLM | |
650 | 7 | |a Dynactin Complex |2 NLM | |
650 | 7 | |a Microtubule-Associated Proteins |2 NLM | |
650 | 7 | |a SNX1 protein, human |2 NLM | |
650 | 7 | |a SNX6 protein, human |2 NLM | |
650 | 7 | |a Sorting Nexins |2 NLM | |
650 | 7 | |a Vesicular Transport Proteins |2 NLM | |
650 | 7 | |a Dyneins |2 NLM | |
650 | 7 | |a EC 3.6.4.2 |2 NLM | |
700 | 1 | |a Yang, Yanrui |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Cheng |e verfasserin |4 aut | |
700 | 1 | |a Niu, Yang |e verfasserin |4 aut | |
700 | 1 | |a Li, Ke |e verfasserin |4 aut | |
700 | 1 | |a Zhao, Xi |e verfasserin |4 aut | |
700 | 1 | |a Liu, Jia-Jia |e verfasserin |4 aut | |
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