Anti-tumor CD8+ T cell immunity elicited by HIV-1-based virus-like particles incorporating HPV-16 E7 protein
Here we report a novel strategy for the induction of CD8(+) T cell adaptive immune response against viral and tumor antigens. This approach relies on high levels of incorporation in HIV-1 VLPs of a mutant of HIV-1 Nef (Nef(mut)) which can act as anchoring element for foreign proteins. By in vitro assay, we found that VLP-associated Nef(mut) is efficiently cross-presented by antigen presenting cells. Inoculation in mice of VLPs incorporating the HPV-16 E7 protein fused to Nef(mut) led to an anti-E7 CD8(+) T cell response much stronger than that elicited by E7 recombinant protein inoculated with incomplete Freund's adjuvant and correlating with well-detectable anti-E7 CTL activity. Most relevantly, mice immunized with Nef(mut)-E7 VLPs developed a protective immune response against tumors induced by E7 expressing tumor cells. These results make Nef(mut) VLPs a promising candidate for new vaccine strategies focused on the induction of CD8(+) T cell immunity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:395 |
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| Enthalten in: |
Virology - 395(2009), 1 vom: 05. Dez., Seite 45-55 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Di Bonito, Paola [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 24.11.2009 Date Revised 18.11.2010 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.virol.2009.09.012 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM191792675 |
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| 245 | 1 | 0 | |a Anti-tumor CD8+ T cell immunity elicited by HIV-1-based virus-like particles incorporating HPV-16 E7 protein |
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| 500 | |a Date Revised 18.11.2010 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Here we report a novel strategy for the induction of CD8(+) T cell adaptive immune response against viral and tumor antigens. This approach relies on high levels of incorporation in HIV-1 VLPs of a mutant of HIV-1 Nef (Nef(mut)) which can act as anchoring element for foreign proteins. By in vitro assay, we found that VLP-associated Nef(mut) is efficiently cross-presented by antigen presenting cells. Inoculation in mice of VLPs incorporating the HPV-16 E7 protein fused to Nef(mut) led to an anti-E7 CD8(+) T cell response much stronger than that elicited by E7 recombinant protein inoculated with incomplete Freund's adjuvant and correlating with well-detectable anti-E7 CTL activity. Most relevantly, mice immunized with Nef(mut)-E7 VLPs developed a protective immune response against tumors induced by E7 expressing tumor cells. These results make Nef(mut) VLPs a promising candidate for new vaccine strategies focused on the induction of CD8(+) T cell immunity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Cancer Vaccines |2 NLM | |
| 650 | 7 | |a G protein, vesicular stomatitis virus |2 NLM | |
| 650 | 7 | |a Membrane Glycoproteins |2 NLM | |
| 650 | 7 | |a Oncogene Proteins, Viral |2 NLM | |
| 650 | 7 | |a Papillomavirus E7 Proteins |2 NLM | |
| 650 | 7 | |a Papillomavirus Vaccines |2 NLM | |
| 650 | 7 | |a Recombinant Fusion Proteins |2 NLM | |
| 650 | 7 | |a Viral Envelope Proteins |2 NLM | |
| 650 | 7 | |a nef Gene Products, Human Immunodeficiency Virus |2 NLM | |
| 650 | 7 | |a nef protein, Human immunodeficiency virus 1 |2 NLM | |
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| 700 | 1 | |a Grasso, Felicia |e verfasserin |4 aut | |
| 700 | 1 | |a Mochi, Stefania |e verfasserin |4 aut | |
| 700 | 1 | |a Petrone, Linda |e verfasserin |4 aut | |
| 700 | 1 | |a Fanales-Belasio, Emanuele |e verfasserin |4 aut | |
| 700 | 1 | |a Mei, Arianna |e verfasserin |4 aut | |
| 700 | 1 | |a Cesolini, Armando |e verfasserin |4 aut | |
| 700 | 1 | |a Laconi, Giuseppe |e verfasserin |4 aut | |
| 700 | 1 | |a Conrad, Heinke |e verfasserin |4 aut | |
| 700 | 1 | |a Bernhard, Helga |e verfasserin |4 aut | |
| 700 | 1 | |a Dembek, Claudia J |e verfasserin |4 aut | |
| 700 | 1 | |a Cosma, Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Santini, Stefano M |e verfasserin |4 aut | |
| 700 | 1 | |a Lapenta, Caterina |e verfasserin |4 aut | |
| 700 | 1 | |a Donati, Simona |e verfasserin |4 aut | |
| 700 | 1 | |a Muratori, Claudia |e verfasserin |4 aut | |
| 700 | 1 | |a Giorgi, Colomba |e verfasserin |4 aut | |
| 700 | 1 | |a Federico, Maurizio |e verfasserin |4 aut | |
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