Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10 degrees C with electrolyte free medium
PURPOSE: In-situ preservation of cauda epididymal spermatozoa at -10 degrees C with electrolyte free media for obtaining maximum functional gametes than preservation at 5 degrees C.
METHODS: Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 - glycerol and soybean lecithin - Coenzyme Q10- glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5 degrees C, wrapped with aluminium foil and freezed at - 10 degrees C. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5 degrees C in a water bath for 5 min.
RESULTS: Spermatozoan characteristics were diminished gradually and significantly (p < 0.001, p < 0.05) between the media and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, viability, hypo-osmotic swelling positivity were 30.2 +/- 0.62; 45.2 +/- 0.82 and 41.6 +/- 0.79 percent respectively on day 21.
CONCLUSION: This method can be adopted in field conditions for transportation of frozen epididymides and re-utilization of maximum functional gametes to conserve valuable animals after postmortem / slaughter.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2009 |
|---|---|
| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
|---|---|
| Enthalten in: |
Journal of assisted reproduction and genetics - 26(2009), 8 vom: 08. Aug., Seite 467-73 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Datta, Uttam [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
1339-63-5 |
|---|
| Anmerkungen: |
Date Completed 06.04.2010 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s10815-009-9344-4 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM191408816 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM191408816 | ||
| 003 | DE-627 | ||
| 005 | 20231227124117.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231223s2009 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10815-009-9344-4 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1222.xml |
| 035 | |a (DE-627)NLM191408816 | ||
| 035 | |a (NLM)19760167 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Datta, Uttam |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Development of a new method to preserve caprine cauda epididymal spermatozoa in-situ at -10 degrees C with electrolyte free medium |
| 264 | 1 | |c 2009 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 06.04.2010 | ||
| 500 | |a Date Revised 13.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a PURPOSE: In-situ preservation of cauda epididymal spermatozoa at -10 degrees C with electrolyte free media for obtaining maximum functional gametes than preservation at 5 degrees C | ||
| 520 | |a METHODS: Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 - glycerol and soybean lecithin - Coenzyme Q10- glycerol were inoculated separately into ligated cauda epididymides, equilibrated 2 h at 5 degrees C, wrapped with aluminium foil and freezed at - 10 degrees C. Spermatozoan characters were evaluated 7 and 21 days after thawing at 38.5 degrees C in a water bath for 5 min | ||
| 520 | |a RESULTS: Spermatozoan characteristics were diminished gradually and significantly (p < 0.001, p < 0.05) between the media and observation days. Soybean lecithin-CoenzymeQ10-glycerol effectively protected spermatozoa against cold shock where spermatozoan progressive motility, viability, hypo-osmotic swelling positivity were 30.2 +/- 0.62; 45.2 +/- 0.82 and 41.6 +/- 0.79 percent respectively on day 21 | ||
| 520 | |a CONCLUSION: This method can be adopted in field conditions for transportation of frozen epididymides and re-utilization of maximum functional gametes to conserve valuable animals after postmortem / slaughter | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Cryoprotective Agents |2 NLM | |
| 650 | 7 | |a Culture Media |2 NLM | |
| 650 | 7 | |a Electrolytes |2 NLM | |
| 650 | 7 | |a Lecithins |2 NLM | |
| 650 | 7 | |a Ubiquinone |2 NLM | |
| 650 | 7 | |a 1339-63-5 |2 NLM | |
| 650 | 7 | |a coenzyme Q10 |2 NLM | |
| 650 | 7 | |a EJ27X76M46 |2 NLM | |
| 650 | 7 | |a Glycerol |2 NLM | |
| 650 | 7 | |a PDC6A3C0OX |2 NLM | |
| 700 | 1 | |a Chandra Sekar, M |e verfasserin |4 aut | |
| 700 | 1 | |a Hembram, Manik Lal |e verfasserin |4 aut | |
| 700 | 1 | |a Dasgupta, Raju |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of assisted reproduction and genetics |d 1996 |g 26(2009), 8 vom: 08. Aug., Seite 467-73 |w (DE-627)NLM012632465 |x 1573-7330 |7 nnns |
| 773 | 1 | 8 | |g volume:26 |g year:2009 |g number:8 |g day:08 |g month:08 |g pages:467-73 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s10815-009-9344-4 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 26 |j 2009 |e 8 |b 08 |c 08 |h 467-73 | ||