Autocrine and paracrine angiopoietin 1/Tie-2 signaling promotes muscle satellite cell self-renewal
Mechanisms governing muscle satellite cell withdrawal from cell cycle to enter into quiescence remain poorly understood. We studied the role of angiopoietin 1 (Ang1) and its receptor Tie-2 in the regulation of myogenic precursor cell (mpc) fate. In human and mouse, Tie-2 was preferentially expressed by quiescent satellite cells in vivo and reserve cells (RCs) in vitro. Ang1/Tie-2 signaling, through ERK1/2 pathway, decreased mpc proliferation and differentiation, increased the number of cells in G0, increased expression of RC-associated markers (p130, Pax7, Myf-5, M-cadherin), and downregulated expression of differentiation-associated markers. Silencing Tie-2 had opposite effects. Cells located in the satellite cell neighborhood (smooth muscle cells, fibroblasts) upregulated RC-associated markers by secreting Ang1 in vitro. In vivo, Tie-2 blockade and Ang1 overexpression increased the number of cycling and quiescent satellite cells, respectively. We propose that Ang1/Tie-2 signaling regulates mpc self-renewal by controlling the return to quiescence of a subset of satellite cells.
| Errataetall: |
CommentIn: Cell Stem Cell. 2009 Sep 4;5(3):231-2. - PMID 19733529 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:5 |
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| Enthalten in: |
Cell stem cell - 5(2009), 3 vom: 04. Sept., Seite 298-309 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Abou-Khalil, Rana [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 04.11.2009 Date Revised 20.10.2021 published: Print CommentIn: Cell Stem Cell. 2009 Sep 4;5(3):231-2. - PMID 19733529 Citation Status MEDLINE |
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| doi: |
10.1016/j.stem.2009.06.001 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM191168289 |
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| 245 | 1 | 0 | |a Autocrine and paracrine angiopoietin 1/Tie-2 signaling promotes muscle satellite cell self-renewal |
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| 500 | |a CommentIn: Cell Stem Cell. 2009 Sep 4;5(3):231-2. - PMID 19733529 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Mechanisms governing muscle satellite cell withdrawal from cell cycle to enter into quiescence remain poorly understood. We studied the role of angiopoietin 1 (Ang1) and its receptor Tie-2 in the regulation of myogenic precursor cell (mpc) fate. In human and mouse, Tie-2 was preferentially expressed by quiescent satellite cells in vivo and reserve cells (RCs) in vitro. Ang1/Tie-2 signaling, through ERK1/2 pathway, decreased mpc proliferation and differentiation, increased the number of cells in G0, increased expression of RC-associated markers (p130, Pax7, Myf-5, M-cadherin), and downregulated expression of differentiation-associated markers. Silencing Tie-2 had opposite effects. Cells located in the satellite cell neighborhood (smooth muscle cells, fibroblasts) upregulated RC-associated markers by secreting Ang1 in vitro. In vivo, Tie-2 blockade and Ang1 overexpression increased the number of cycling and quiescent satellite cells, respectively. We propose that Ang1/Tie-2 signaling regulates mpc self-renewal by controlling the return to quiescence of a subset of satellite cells | ||
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| 700 | 1 | |a Pallafacchina, Giorgia |e verfasserin |4 aut | |
| 700 | 1 | |a Valable, Samuel |e verfasserin |4 aut | |
| 700 | 1 | |a Authier, François-Jérôme |e verfasserin |4 aut | |
| 700 | 1 | |a Rudnicki, Michael A |e verfasserin |4 aut | |
| 700 | 1 | |a Gherardi, Romain K |e verfasserin |4 aut | |
| 700 | 1 | |a Germain, Stéphane |e verfasserin |4 aut | |
| 700 | 1 | |a Chretien, Fabrice |e verfasserin |4 aut | |
| 700 | 1 | |a Sotiropoulos, Athanassia |e verfasserin |4 aut | |
| 700 | 1 | |a Lafuste, Peggy |e verfasserin |4 aut | |
| 700 | 1 | |a Montarras, Didier |e verfasserin |4 aut | |
| 700 | 1 | |a Chazaud, Bénédicte |e verfasserin |4 aut | |
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