The OsDHODH1 gene is involved in salt and drought tolerance in rice
In the present paper, we identified and cloned OsDHODH1 encoding a putative cytosolic dihydroorotate dehydrogenase (DHODH) in rice. Expression analysis indicated that OsDHODH1 is upregulated by salt, drought and exogenous abscisic acid (ABA), but not by cold. By prokaryotic expression, we determined the enzymatic activity of OsDHODH1 and found that overproduction of OsDHODH1 significantly improved the tolerance of Escherichia coli cells to salt and osmotic stresses. Overexpression of the OsDHODH1 gene in rice increased the DHODH activity and enhanced plant tolerance to salt and drought stresses as compared with wild type and OsDHODH1-antisense transgenic plants. Our findings reveal, for the first time, that cytosolic dihydroorotate dehydrogenase is involved in plant stress response and that OsDHODH1 could be used in engineering crop plants with enhanced tolerance to salt and drought.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2009 |
|---|---|
| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:51 |
|---|---|
| Enthalten in: |
Journal of integrative plant biology - 51(2009), 9 vom: 15. Sept., Seite 825-33 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Liu, Wen-Ying [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 13.11.2009 Date Revised 08.04.2022 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1111/j.1744-7909.2009.00853.x |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM191071595 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM191071595 | ||
| 003 | DE-627 | ||
| 005 | 20231223190526.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231223s2009 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/j.1744-7909.2009.00853.x |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0637.xml |
| 035 | |a (DE-627)NLM191071595 | ||
| 035 | |a (NLM)19723241 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Liu, Wen-Ying |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The OsDHODH1 gene is involved in salt and drought tolerance in rice |
| 264 | 1 | |c 2009 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 13.11.2009 | ||
| 500 | |a Date Revised 08.04.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a In the present paper, we identified and cloned OsDHODH1 encoding a putative cytosolic dihydroorotate dehydrogenase (DHODH) in rice. Expression analysis indicated that OsDHODH1 is upregulated by salt, drought and exogenous abscisic acid (ABA), but not by cold. By prokaryotic expression, we determined the enzymatic activity of OsDHODH1 and found that overproduction of OsDHODH1 significantly improved the tolerance of Escherichia coli cells to salt and osmotic stresses. Overexpression of the OsDHODH1 gene in rice increased the DHODH activity and enhanced plant tolerance to salt and drought stresses as compared with wild type and OsDHODH1-antisense transgenic plants. Our findings reveal, for the first time, that cytosolic dihydroorotate dehydrogenase is involved in plant stress response and that OsDHODH1 could be used in engineering crop plants with enhanced tolerance to salt and drought | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Dihydroorotate Dehydrogenase |2 NLM | |
| 650 | 7 | |a Oxidants |2 NLM | |
| 650 | 7 | |a Plant Growth Regulators |2 NLM | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 650 | 7 | |a Chlorophyll |2 NLM | |
| 650 | 7 | |a 1406-65-1 |2 NLM | |
| 650 | 7 | |a Sodium Chloride |2 NLM | |
| 650 | 7 | |a 451W47IQ8X |2 NLM | |
| 650 | 7 | |a Abscisic Acid |2 NLM | |
| 650 | 7 | |a 72S9A8J5GW |2 NLM | |
| 650 | 7 | |a Proline |2 NLM | |
| 650 | 7 | |a 9DLQ4CIU6V |2 NLM | |
| 650 | 7 | |a Hydrogen Peroxide |2 NLM | |
| 650 | 7 | |a BBX060AN9V |2 NLM | |
| 650 | 7 | |a Oxidoreductases Acting on CH-CH Group Donors |2 NLM | |
| 650 | 7 | |a EC 1.3.- |2 NLM | |
| 700 | 1 | |a Wang, Mei-Mei |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Ji |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Hai-Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Lan, Hong-Xia |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hong-Sheng |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of integrative plant biology |d 2008 |g 51(2009), 9 vom: 15. Sept., Seite 825-33 |w (DE-627)NLM181258781 |x 1744-7909 |7 nnns |
| 773 | 1 | 8 | |g volume:51 |g year:2009 |g number:9 |g day:15 |g month:09 |g pages:825-33 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/j.1744-7909.2009.00853.x |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 51 |j 2009 |e 9 |b 15 |c 09 |h 825-33 | ||