Downregulation of miRNA-200c links breast cancer stem cells with normal stem cells
Human breast tumors contain a breast cancer stem cell (BCSC) population with properties reminiscent of normal stem cells. We found 37 microRNAs that were differentially expressed between human BCSCs and nontumorigenic cancer cells. Three clusters, miR-200c-141, miR-200b-200a-429, and miR-183-96-182 were downregulated in human BCSCs, normal human and murine mammary stem/progenitor cells, and embryonal carcinoma cells. Expression of BMI1, a known regulator of stem cell self-renewal, was modulated by miR-200c. miR-200c inhibited the clonal expansion of breast cancer cells and suppressed the growth of embryonal carcinoma cells in vitro. Most importantly, miR-200c strongly suppressed the ability of normal mammary stem cells to form mammary ducts and tumor formation driven by human BCSCs in vivo. The coordinated downregulation of three microRNA clusters and the similar functional regulation of clonal expansion by miR-200c provide a molecular link that connects BCSCs with normal stem cells.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:138 |
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| Enthalten in: |
Cell - 138(2009), 3 vom: 07. Aug., Seite 592-603 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shimono, Yohei [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 31.08.2009 Date Revised 09.04.2022 published: Print CommentIn: Cell. 2009 Aug 7;138(3):423-4. - PMID 19665962 Citation Status MEDLINE |
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| doi: |
10.1016/j.cell.2009.07.011 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM190555211 |
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| 100 | 1 | |a Shimono, Yohei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Downregulation of miRNA-200c links breast cancer stem cells with normal stem cells |
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| 500 | |a CommentIn: Cell. 2009 Aug 7;138(3):423-4. - PMID 19665962 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Human breast tumors contain a breast cancer stem cell (BCSC) population with properties reminiscent of normal stem cells. We found 37 microRNAs that were differentially expressed between human BCSCs and nontumorigenic cancer cells. Three clusters, miR-200c-141, miR-200b-200a-429, and miR-183-96-182 were downregulated in human BCSCs, normal human and murine mammary stem/progenitor cells, and embryonal carcinoma cells. Expression of BMI1, a known regulator of stem cell self-renewal, was modulated by miR-200c. miR-200c inhibited the clonal expansion of breast cancer cells and suppressed the growth of embryonal carcinoma cells in vitro. Most importantly, miR-200c strongly suppressed the ability of normal mammary stem cells to form mammary ducts and tumor formation driven by human BCSCs in vivo. The coordinated downregulation of three microRNA clusters and the similar functional regulation of clonal expansion by miR-200c provide a molecular link that connects BCSCs with normal stem cells | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Zabala, Maider |e verfasserin |4 aut | |
| 700 | 1 | |a Cho, Robert W |e verfasserin |4 aut | |
| 700 | 1 | |a Lobo, Neethan |e verfasserin |4 aut | |
| 700 | 1 | |a Dalerba, Piero |e verfasserin |4 aut | |
| 700 | 1 | |a Qian, Dalong |e verfasserin |4 aut | |
| 700 | 1 | |a Diehn, Maximilian |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Huiping |e verfasserin |4 aut | |
| 700 | 1 | |a Panula, Sarita P |e verfasserin |4 aut | |
| 700 | 1 | |a Chiao, Eric |e verfasserin |4 aut | |
| 700 | 1 | |a Dirbas, Frederick M |e verfasserin |4 aut | |
| 700 | 1 | |a Somlo, George |e verfasserin |4 aut | |
| 700 | 1 | |a Pera, Renee A Reijo |e verfasserin |4 aut | |
| 700 | 1 | |a Lao, Kaiqin |e verfasserin |4 aut | |
| 700 | 1 | |a Clarke, Michael F |e verfasserin |4 aut | |
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