Anti-angiogenic effects and mechanisms of polysaccharides from Antrodia cinnamomea with different molecular weights
ETHNOPHARMACOLOGICAL RELEVANCE: Antrodia cinnamomea is a popular medicinal mushroom in Taiwan that has been widely used for treatment of various cancers and liver diseases.
AIM OF THE STUDY: This study aimed to investigate the immunomodulatory effect on angiogenesis of polysaccharides from mycelia of Antrodia cinnamomea (PMAC).
MATERIALS AND METHODS: PMAC were extracted in boiling water, precipitated with 95% ethanol, and separated into four different molecular weights (<5, 5-30, 30-100, > 100 kDa). Tube formation and chorioallantoic membrane (CAM) assay were used to determine the in vitro and ex vivo anti-angiogenic effects.
RESULTS: Only the PMAC-mononuclear cells (MNCs)-conditioned medium (CM) with MW > 100 kDa significantly and concentration-dependently decreased the secretion of vascular endothelial growth factor in human leukemia cells and inhibited the matrigel tube formation in human umbilical vein endothelial cells. Similarly only the PMAC-MNC-CM with MW > 100 kDa significantly and concentration-dependently increased the levels of interleukin (IL)-12 and interferon-gamma (IFN-gamma). In addition, the ex vivo CAM assay revealed that only the PMAC with MW>100 kDa significantly and dose-dependently inhibited neovascularization.
CONCLUSIONS: PMAC with MW > 100 kDa are anti-angiogenic in vitro and ex vivo, and the effects are likely through immunomodulation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:123 |
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| Enthalten in: |
Journal of ethnopharmacology - 123(2009), 3 vom: 25. Juni, Seite 407-12 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yang, Chih-Min [VerfasserIn] |
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| Links: |
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| Themen: |
187348-17-0 |
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| Anmerkungen: |
Date Completed 08.10.2009 Date Revised 01.12.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jep.2009.03.034 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM189081864 |
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| 100 | 1 | |a Yang, Chih-Min |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Anti-angiogenic effects and mechanisms of polysaccharides from Antrodia cinnamomea with different molecular weights |
| 264 | 1 | |c 2009 | |
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| 500 | |a Date Completed 08.10.2009 | ||
| 500 | |a Date Revised 01.12.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a ETHNOPHARMACOLOGICAL RELEVANCE: Antrodia cinnamomea is a popular medicinal mushroom in Taiwan that has been widely used for treatment of various cancers and liver diseases | ||
| 520 | |a AIM OF THE STUDY: This study aimed to investigate the immunomodulatory effect on angiogenesis of polysaccharides from mycelia of Antrodia cinnamomea (PMAC) | ||
| 520 | |a MATERIALS AND METHODS: PMAC were extracted in boiling water, precipitated with 95% ethanol, and separated into four different molecular weights (<5, 5-30, 30-100, > 100 kDa). Tube formation and chorioallantoic membrane (CAM) assay were used to determine the in vitro and ex vivo anti-angiogenic effects | ||
| 520 | |a RESULTS: Only the PMAC-mononuclear cells (MNCs)-conditioned medium (CM) with MW > 100 kDa significantly and concentration-dependently decreased the secretion of vascular endothelial growth factor in human leukemia cells and inhibited the matrigel tube formation in human umbilical vein endothelial cells. Similarly only the PMAC-MNC-CM with MW > 100 kDa significantly and concentration-dependently increased the levels of interleukin (IL)-12 and interferon-gamma (IFN-gamma). In addition, the ex vivo CAM assay revealed that only the PMAC with MW>100 kDa significantly and dose-dependently inhibited neovascularization | ||
| 520 | |a CONCLUSIONS: PMAC with MW > 100 kDa are anti-angiogenic in vitro and ex vivo, and the effects are likely through immunomodulation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Angiogenesis Inhibitors |2 NLM | |
| 650 | 7 | |a Immunologic Factors |2 NLM | |
| 650 | 7 | |a Plant Extracts |2 NLM | |
| 650 | 7 | |a Polysaccharides |2 NLM | |
| 650 | 7 | |a Vascular Endothelial Growth Factor A |2 NLM | |
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| 700 | 1 | |a Zhou, Yu-Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ran-Juh |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Miao-Lin |e verfasserin |4 aut | |
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