Antioxidant and anti-apoptotic effect of melatonin on the vestibular hair cells of rat utricles
OBJECTIVES: Aminoglycosides are commonly used antibiotic agents, and they are known to generate free oxygen radicals within the inner ear and to cause vestibulo-cochlear toxicity and permanent damage to the sensory hair cells and neurons. Melatonin, a pineal secretory product, has the properties of being a powerful direct and indirect antioxidant. The aim of the present study was to prove the antioxidant effect of melatonin against gentamicin-induced ototoxicty.
METHODS: The utricular maculae of Sprague-Dawley rats were prepared from postnatal day 2-4, and these maculae were were divided into 6 groups as follows: 1) control, 2) melatonin only, 3) gentamicin only, and 4), 5), and 6) gentamicin plus melatonin (10, 50, and 100 microM, respectively). To count the number of hair cells, 5 utricles from each group were stained with phalloidin-FITC on the 1st, 4th, and 7th days after drug administration. Reactive oxygen species (ROS) was assessed by using the fluorescent probe hydrofluorescent diacetate acetyl ester. The caspase-3 activity was also examined with using the fluorescent caspase-3 substrate and performing Western blotting.
RESULTS: The result of this study showed that gentamicin induced the loss of utricular hair cells, and this loss of hair cells was significantly attenuated by co-administration of melatonin. Melatonin reduced ROS production and caspase-3 activation in the gentamicin treated utricular hair cells.
CONCLUSION: Our findings conclusively reveal that melatonin has protective effects against gentamicin-induced hair cell loss in the utricles of rat by inhibiting both ROS production and caspase-3 activity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
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| Enthalten in: |
Clinical and experimental otorhinolaryngology - 2(2009), 1 vom: 05. März, Seite 6-12 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kim, Jeong-Beom [VerfasserIn] |
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| Themen: |
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| Anmerkungen: |
Date Completed 14.07.2011 Date Revised 20.10.2021 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3342/ceo.2009.2.1.6 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM18845134X |
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| 100 | 1 | |a Kim, Jeong-Beom |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Antioxidant and anti-apoptotic effect of melatonin on the vestibular hair cells of rat utricles |
| 264 | 1 | |c 2009 | |
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| 500 | |a Date Completed 14.07.2011 | ||
| 500 | |a Date Revised 20.10.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a OBJECTIVES: Aminoglycosides are commonly used antibiotic agents, and they are known to generate free oxygen radicals within the inner ear and to cause vestibulo-cochlear toxicity and permanent damage to the sensory hair cells and neurons. Melatonin, a pineal secretory product, has the properties of being a powerful direct and indirect antioxidant. The aim of the present study was to prove the antioxidant effect of melatonin against gentamicin-induced ototoxicty | ||
| 520 | |a METHODS: The utricular maculae of Sprague-Dawley rats were prepared from postnatal day 2-4, and these maculae were were divided into 6 groups as follows: 1) control, 2) melatonin only, 3) gentamicin only, and 4), 5), and 6) gentamicin plus melatonin (10, 50, and 100 microM, respectively). To count the number of hair cells, 5 utricles from each group were stained with phalloidin-FITC on the 1st, 4th, and 7th days after drug administration. Reactive oxygen species (ROS) was assessed by using the fluorescent probe hydrofluorescent diacetate acetyl ester. The caspase-3 activity was also examined with using the fluorescent caspase-3 substrate and performing Western blotting | ||
| 520 | |a RESULTS: The result of this study showed that gentamicin induced the loss of utricular hair cells, and this loss of hair cells was significantly attenuated by co-administration of melatonin. Melatonin reduced ROS production and caspase-3 activation in the gentamicin treated utricular hair cells | ||
| 520 | |a CONCLUSION: Our findings conclusively reveal that melatonin has protective effects against gentamicin-induced hair cell loss in the utricles of rat by inhibiting both ROS production and caspase-3 activity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antioxidants | |
| 650 | 4 | |a Melatonin | |
| 650 | 4 | |a Ototoxicity | |
| 650 | 4 | |a Utricle | |
| 700 | 1 | |a Jung, Jae Yun |e verfasserin |4 aut | |
| 700 | 1 | |a Ahn, Jin-Chul |e verfasserin |4 aut | |
| 700 | 1 | |a Rhee, Chung Ku |e verfasserin |4 aut | |
| 700 | 1 | |a Hwang, Hee-Jun |e verfasserin |4 aut | |
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