The fluorescence enhancement of quercetin-nucleic acid system and the analytical application
Nucleic acid can greatly enhance the fluorescence intensity of quercetin in HMTA-HCl (pH 5.5) buffer. The enhanced intensity is in proportion to the concentration of nucleic acids in the range 5.0 x 10(-9) to 1.0 x 10(-6) g/mL for fsDNA, 5.0 x 10(-9) to 7.0 x 10(-7) g/mL for ctDNA and 5.0 x 10(-9) to 1.0 x 10(-6) g/mL for yRNA, and their detection limits (S/N = 3) are 3.5 x 10(-9), 7.8 x 10(-10) and 2.6 x 10(-9) g/mL, respectively. In comparison with most reported fluorescent probes for the determination of nucleic acids, the proposed probe has higher sensitivity and lower toxicity. The interaction investigation indicates that quercetin binds with double-strand DNA in groove binding mode, resulting in fluorescence enhancement of this system.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
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| Enthalten in: |
Luminescence : the journal of biological and chemical luminescence - 24(2009), 6 vom: 30. Nov., Seite 416-21 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Yuan [VerfasserIn] |
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| Links: |
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| Themen: |
9IKM0I5T1E |
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| Anmerkungen: |
Date Completed 31.03.2010 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
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| doi: |
10.1002/bio.1128 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM188363505 |
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| 245 | 1 | 4 | |a The fluorescence enhancement of quercetin-nucleic acid system and the analytical application |
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| 500 | |a Date Completed 31.03.2010 | ||
| 500 | |a Date Revised 21.11.2013 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Nucleic acid can greatly enhance the fluorescence intensity of quercetin in HMTA-HCl (pH 5.5) buffer. The enhanced intensity is in proportion to the concentration of nucleic acids in the range 5.0 x 10(-9) to 1.0 x 10(-6) g/mL for fsDNA, 5.0 x 10(-9) to 7.0 x 10(-7) g/mL for ctDNA and 5.0 x 10(-9) to 1.0 x 10(-6) g/mL for yRNA, and their detection limits (S/N = 3) are 3.5 x 10(-9), 7.8 x 10(-10) and 2.6 x 10(-9) g/mL, respectively. In comparison with most reported fluorescent probes for the determination of nucleic acids, the proposed probe has higher sensitivity and lower toxicity. The interaction investigation indicates that quercetin binds with double-strand DNA in groove binding mode, resulting in fluorescence enhancement of this system | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Fluorescent Dyes |2 NLM | |
| 650 | 7 | |a Nucleic Acids |2 NLM | |
| 650 | 7 | |a Quercetin |2 NLM | |
| 650 | 7 | |a 9IKM0I5T1E |2 NLM | |
| 700 | 1 | |a Wu, Xia |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Haiping |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaoyu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Fengju |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Jinghe |e verfasserin |4 aut | |
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