Intragraft FOXP3 protein or mRNA during acute renal allograft rejection correlates with inflammation, fibrosis, and poor renal outcome
BACKGROUND: Forkhead box (FOXP3) is considered to be a specific marker for regulatory T cells. The aim of this study was to correlate intragraft FOXP3 at mRNA and cellular levels during renal allograft rejection to response to therapy and late clinical outcome.
METHODS: Immunostainings and quantitative reverse-transcriptase polymerase chain reaction for FOXP3, CD3, and transforming growth factor (TGF)-beta were performed and results were related to histopathologic and clinical outcome.
RESULTS: A good correlation between immunohistochemical analysis and mRNA levels for both CD3 and FOXP3 was observed. Intragraft FOXP3 was significantly related to tubulitis and interstitial fibrosis. A strong correlation was found between FOXP3 and CD3 mRNA and between FOXP3 and TGF-beta mRNA. No correlation was found between FOXP3 and response to therapy.
DISCUSSION: In conclusion, intragraft FOXP3 at both cellular and molecular levels parallels T-cell infiltration during acute rejection. FOXP3 does not predict response to antirejection therapy. FOXP3 correlates with renal fibrosis, TGF-beta, and poor late renal outcome.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:87 |
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| Enthalten in: |
Transplantation - 87(2009), 9 vom: 15. Mai, Seite 1377-80 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yapici, Unsal [VerfasserIn] |
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| Links: |
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| Themen: |
Antigens, CD |
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| Anmerkungen: |
Date Completed 08.07.2009 Date Revised 16.11.2017 published: Print Citation Status MEDLINE |
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| doi: |
10.1097/TP.0b013e3181a24a4b |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM188355170 |
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| 100 | 1 | |a Yapici, Unsal |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Intragraft FOXP3 protein or mRNA during acute renal allograft rejection correlates with inflammation, fibrosis, and poor renal outcome |
| 264 | 1 | |c 2009 | |
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| 500 | |a Date Completed 08.07.2009 | ||
| 500 | |a Date Revised 16.11.2017 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Forkhead box (FOXP3) is considered to be a specific marker for regulatory T cells. The aim of this study was to correlate intragraft FOXP3 at mRNA and cellular levels during renal allograft rejection to response to therapy and late clinical outcome | ||
| 520 | |a METHODS: Immunostainings and quantitative reverse-transcriptase polymerase chain reaction for FOXP3, CD3, and transforming growth factor (TGF)-beta were performed and results were related to histopathologic and clinical outcome | ||
| 520 | |a RESULTS: A good correlation between immunohistochemical analysis and mRNA levels for both CD3 and FOXP3 was observed. Intragraft FOXP3 was significantly related to tubulitis and interstitial fibrosis. A strong correlation was found between FOXP3 and CD3 mRNA and between FOXP3 and TGF-beta mRNA. No correlation was found between FOXP3 and response to therapy | ||
| 520 | |a DISCUSSION: In conclusion, intragraft FOXP3 at both cellular and molecular levels parallels T-cell infiltration during acute rejection. FOXP3 does not predict response to antirejection therapy. FOXP3 correlates with renal fibrosis, TGF-beta, and poor late renal outcome | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Antigens, CD |2 NLM | |
| 650 | 7 | |a CD3 Complex |2 NLM | |
| 650 | 7 | |a FOXP3 protein, human |2 NLM | |
| 650 | 7 | |a Forkhead Transcription Factors |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Transforming Growth Factor beta |2 NLM | |
| 700 | 1 | |a Bemelman, Fréderike J |e verfasserin |4 aut | |
| 700 | 1 | |a Scheepstra, Cornelis G |e verfasserin |4 aut | |
| 700 | 1 | |a Roelofs, Joris J T H |e verfasserin |4 aut | |
| 700 | 1 | |a Claessen, Nike |e verfasserin |4 aut | |
| 700 | 1 | |a van der Loos, Chris |e verfasserin |4 aut | |
| 700 | 1 | |a van Donselaar-van der Pant, Karlijn |e verfasserin |4 aut | |
| 700 | 1 | |a Bouts, Antonia H M |e verfasserin |4 aut | |
| 700 | 1 | |a Idu, Mirza M |e verfasserin |4 aut | |
| 700 | 1 | |a Rowshani, Ajda T |e verfasserin |4 aut | |
| 700 | 1 | |a ten Berge, Ineke J M |e verfasserin |4 aut | |
| 700 | 1 | |a Florquin, Sandrine |e verfasserin |4 aut | |
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