Dickkopf-1 enhances migration of HEK293 cell by beta-catenin/E-cadherin degradation
Migration is an important process during cellular activity and embryo development. We recently showed that Dickkopf-1(Dkk-1), an antagonist of Wnt/ beta-catenin signaling pathway, could promote trophoblast cell invasion during murine placentation. However, mechanism of Dkk-1 action on cell migration was not clear. The objective of this study was to further evaluate the effect of Dkk-1 on cell migration and to identify the underlining mechanisms. Functional assays with stable Dkk-1 transfected HEK293 cells revealed that Dkk-1 expression increased cell migration by decreasing cell-cell adhesion, not cell-matrix adhesion. Treatment with LiCl and Genistein (widely used inhibitor of glycogen synthase kinase-3 and tyrosine protein kinase, respectively.) could inhibit the migration effect of Dkk-1, and significantly increased the membrane localization of beta-catenin and E-cadherin in HEK293 cells transfected with Dkk-1. Further data showed that HEK293 cells transfected with Dkk-1 have significantly decreased accumulation of both beta-catenin and E-cadherin at the cell membrane. Together, our data suggest that Dkk-1 stimulates the release of beta-catenin from cell membrane and facilitates cell migration which accompanies degradation of beta-catenin/E-cadherin.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2009 |
---|---|
Erschienen: |
2009 |
Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
---|---|
Enthalten in: |
Frontiers in bioscience (Landmark edition) - 14(2009), 6 vom: 01. Jan., Seite 2212-20 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Kuang, Hai-Bin [VerfasserIn] |
---|
Links: |
---|
Themen: |
Beta Catenin |
---|
Anmerkungen: |
Date Completed 07.04.2009 Date Revised 27.02.2022 published: Electronic Citation Status MEDLINE |
---|
doi: |
10.2741/3373 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM186960670 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM186960670 | ||
003 | DE-627 | ||
005 | 20231223175119.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231223s2009 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.2741/3373 |2 doi | |
028 | 5 | 2 | |a pubmed24n0623.xml |
035 | |a (DE-627)NLM186960670 | ||
035 | |a (NLM)19273195 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Kuang, Hai-Bin |e verfasserin |4 aut | |
245 | 1 | 0 | |a Dickkopf-1 enhances migration of HEK293 cell by beta-catenin/E-cadherin degradation |
264 | 1 | |c 2009 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 07.04.2009 | ||
500 | |a Date Revised 27.02.2022 | ||
500 | |a published: Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Migration is an important process during cellular activity and embryo development. We recently showed that Dickkopf-1(Dkk-1), an antagonist of Wnt/ beta-catenin signaling pathway, could promote trophoblast cell invasion during murine placentation. However, mechanism of Dkk-1 action on cell migration was not clear. The objective of this study was to further evaluate the effect of Dkk-1 on cell migration and to identify the underlining mechanisms. Functional assays with stable Dkk-1 transfected HEK293 cells revealed that Dkk-1 expression increased cell migration by decreasing cell-cell adhesion, not cell-matrix adhesion. Treatment with LiCl and Genistein (widely used inhibitor of glycogen synthase kinase-3 and tyrosine protein kinase, respectively.) could inhibit the migration effect of Dkk-1, and significantly increased the membrane localization of beta-catenin and E-cadherin in HEK293 cells transfected with Dkk-1. Further data showed that HEK293 cells transfected with Dkk-1 have significantly decreased accumulation of both beta-catenin and E-cadherin at the cell membrane. Together, our data suggest that Dkk-1 stimulates the release of beta-catenin from cell membrane and facilitates cell migration which accompanies degradation of beta-catenin/E-cadherin | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a Cadherins |2 NLM | |
650 | 7 | |a DKK1 protein, human |2 NLM | |
650 | 7 | |a Intercellular Signaling Peptides and Proteins |2 NLM | |
650 | 7 | |a beta Catenin |2 NLM | |
650 | 7 | |a Genistein |2 NLM | |
650 | 7 | |a DH2M523P0H |2 NLM | |
650 | 7 | |a Lithium Chloride |2 NLM | |
650 | 7 | |a G4962QA067 |2 NLM | |
700 | 1 | |a Miao, Cheng-Lin |e verfasserin |4 aut | |
700 | 1 | |a Guo, Wei-Xiang |e verfasserin |4 aut | |
700 | 1 | |a Peng, Sha |e verfasserin |4 aut | |
700 | 1 | |a Cao, Yu-Jing |e verfasserin |4 aut | |
700 | 1 | |a Duan, En-Kui |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Frontiers in bioscience (Landmark edition) |d 2009 |g 14(2009), 6 vom: 01. Jan., Seite 2212-20 |w (DE-627)NLM186959230 |x 2768-6698 |7 nnns |
773 | 1 | 8 | |g volume:14 |g year:2009 |g number:6 |g day:01 |g month:01 |g pages:2212-20 |
856 | 4 | 0 | |u http://dx.doi.org/10.2741/3373 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 14 |j 2009 |e 6 |b 01 |c 01 |h 2212-20 |