Activation of CD47 receptors causes histamine secretion from mast cells
Mast cells play pivotal roles in allergic and inflammatory processes via distinct activation pathways. Mucosal and serosal mast cells are activated by the IgE/FcepsilonRI pathway, while only serosal mast cells are activated by basic secretagogues. We show that CD47 receptors are expressed on rat peritoneal mast cells. 4N1K, a peptide agonist of CD47, rapidly caused exocytosis. Such exocytosis required increased intracellular calcium and was inhibited by pertussis toxin and an antibody against the betagamma dimer of a G(i) protein. Cooperation with integrins and glycosylphosphatidylinositol-anchored proteins was necessary, since anti-integrin antibodies and pretreatment with phosphatidylinositol-phospholipase C reduced exocytosis. Depletion of membrane cholesterol inhibited exocytosis and decreased CD47 in lipid rafts, consistent with a CD47/integrin/G(i) protein complex being located in rafts. An anti-CD47 antibody inhibited exocytosis induced by 4N1K and by mastoparan and spermine, suggesting that basic secretagogues might target CD47. We propose that 4N1K-stimulated mast cell exocytosis involves a CD47/integrin/G(i) protein complex.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2009 |
|---|---|
| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:66 |
|---|---|
| Enthalten in: |
Cellular and molecular life sciences : CMLS - 66(2009), 7 vom: 01. Apr., Seite 1271-82 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Sick, E [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 16.06.2009 Date Revised 10.12.2019 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1007/s00018-009-8778-2 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM186325355 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM186325355 | ||
| 003 | DE-627 | ||
| 005 | 20231223174018.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231223s2009 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00018-009-8778-2 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0621.xml |
| 035 | |a (DE-627)NLM186325355 | ||
| 035 | |a (NLM)19205621 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Sick, E |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Activation of CD47 receptors causes histamine secretion from mast cells |
| 264 | 1 | |c 2009 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 16.06.2009 | ||
| 500 | |a Date Revised 10.12.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Mast cells play pivotal roles in allergic and inflammatory processes via distinct activation pathways. Mucosal and serosal mast cells are activated by the IgE/FcepsilonRI pathway, while only serosal mast cells are activated by basic secretagogues. We show that CD47 receptors are expressed on rat peritoneal mast cells. 4N1K, a peptide agonist of CD47, rapidly caused exocytosis. Such exocytosis required increased intracellular calcium and was inhibited by pertussis toxin and an antibody against the betagamma dimer of a G(i) protein. Cooperation with integrins and glycosylphosphatidylinositol-anchored proteins was necessary, since anti-integrin antibodies and pretreatment with phosphatidylinositol-phospholipase C reduced exocytosis. Depletion of membrane cholesterol inhibited exocytosis and decreased CD47 in lipid rafts, consistent with a CD47/integrin/G(i) protein complex being located in rafts. An anti-CD47 antibody inhibited exocytosis induced by 4N1K and by mastoparan and spermine, suggesting that basic secretagogues might target CD47. We propose that 4N1K-stimulated mast cell exocytosis involves a CD47/integrin/G(i) protein complex | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a 4N1K peptide |2 NLM | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a CD47 Antigen |2 NLM | |
| 650 | 7 | |a G-protein Beta gamma |2 NLM | |
| 650 | 7 | |a GTP-Binding Protein beta Subunits |2 NLM | |
| 650 | 7 | |a GTP-Binding Protein gamma Subunits |2 NLM | |
| 650 | 7 | |a Glycosylphosphatidylinositols |2 NLM | |
| 650 | 7 | |a Integrin beta Chains |2 NLM | |
| 650 | 7 | |a Intercellular Signaling Peptides and Proteins |2 NLM | |
| 650 | 7 | |a Oligopeptides |2 NLM | |
| 650 | 7 | |a Peptides |2 NLM | |
| 650 | 7 | |a Reactive Oxygen Species |2 NLM | |
| 650 | 7 | |a Wasp Venoms |2 NLM | |
| 650 | 7 | |a Spermine |2 NLM | |
| 650 | 7 | |a 2FZ7Y3VOQX |2 NLM | |
| 650 | 7 | |a mastoparan |2 NLM | |
| 650 | 7 | |a 72093-21-1 |2 NLM | |
| 650 | 7 | |a Histamine |2 NLM | |
| 650 | 7 | |a 820484N8I3 |2 NLM | |
| 650 | 7 | |a Cholesterol |2 NLM | |
| 650 | 7 | |a 97C5T2UQ7J |2 NLM | |
| 650 | 7 | |a Pertussis Toxin |2 NLM | |
| 650 | 7 | |a EC 2.4.2.31 |2 NLM | |
| 650 | 7 | |a Type C Phospholipases |2 NLM | |
| 650 | 7 | |a EC 3.1.4.- |2 NLM | |
| 700 | 1 | |a Niederhoffer, N |e verfasserin |4 aut | |
| 700 | 1 | |a Takeda, K |e verfasserin |4 aut | |
| 700 | 1 | |a Landry, Y |e verfasserin |4 aut | |
| 700 | 1 | |a Gies, J-P |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cellular and molecular life sciences : CMLS |d 1997 |g 66(2009), 7 vom: 01. Apr., Seite 1271-82 |w (DE-627)NLM090610393 |x 1420-9071 |7 nnns |
| 773 | 1 | 8 | |g volume:66 |g year:2009 |g number:7 |g day:01 |g month:04 |g pages:1271-82 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00018-009-8778-2 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 66 |j 2009 |e 7 |b 01 |c 04 |h 1271-82 | ||