Identification of Old World Leishmania spp. by specific polymerase chain reaction amplification of cysteine proteinase B genes and rapid dipstick detection
We used the cysteine proteinase B (cpb) gene family of the trypanosomatid genus Leishmania as a target to develop rapid, specific, and easy-to-use polymerase chain reaction (PCR) tests to discriminate Leishmania infantum, Leishmania donovani, Leishmania tropica, Leishmania aethiopica, and Leishmania major. Identification of all 5 Old World species and validation of intraspecies variability are features lacking in other species-specific PCRs. Amplicon analysis was done on agarose gels and was further simplified by using an oligochromatography dipstick to detect L. infantum and L. donovani products. Because the analytical sensitivity is lower than that of certain other species- and genus-specific PCRs, our assays are especially valuable for use on cultured isolates or directly on cryostabilates. As such, they can be implemented by research and health centers having access to culturing, DNA isolation, and PCR.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2009 |
|---|---|
| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:63 |
|---|---|
| Enthalten in: |
Diagnostic microbiology and infectious disease - 63(2009), 2 vom: 12. Feb., Seite 173-81 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Laurent, Thierry [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Cysteine Endopeptidases |
|---|
| Anmerkungen: |
Date Completed 07.08.2009 Date Revised 19.01.2009 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.diagmicrobio.2008.10.015 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM185317952 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM185317952 | ||
| 003 | DE-627 | ||
| 005 | 20231223172257.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231223s2009 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.diagmicrobio.2008.10.015 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0618.xml |
| 035 | |a (DE-627)NLM185317952 | ||
| 035 | |a (NLM)19097841 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Laurent, Thierry |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification of Old World Leishmania spp. by specific polymerase chain reaction amplification of cysteine proteinase B genes and rapid dipstick detection |
| 264 | 1 | |c 2009 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 07.08.2009 | ||
| 500 | |a Date Revised 19.01.2009 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a We used the cysteine proteinase B (cpb) gene family of the trypanosomatid genus Leishmania as a target to develop rapid, specific, and easy-to-use polymerase chain reaction (PCR) tests to discriminate Leishmania infantum, Leishmania donovani, Leishmania tropica, Leishmania aethiopica, and Leishmania major. Identification of all 5 Old World species and validation of intraspecies variability are features lacking in other species-specific PCRs. Amplicon analysis was done on agarose gels and was further simplified by using an oligochromatography dipstick to detect L. infantum and L. donovani products. Because the analytical sensitivity is lower than that of certain other species- and genus-specific PCRs, our assays are especially valuable for use on cultured isolates or directly on cryostabilates. As such, they can be implemented by research and health centers having access to culturing, DNA isolation, and PCR | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA Primers |2 NLM | |
| 650 | 7 | |a Cysteine Endopeptidases |2 NLM | |
| 650 | 7 | |a EC 3.4.22.- |2 NLM | |
| 700 | 1 | |a Van der Auwera, Gert |e verfasserin |4 aut | |
| 700 | 1 | |a Hide, Mallorie |e verfasserin |4 aut | |
| 700 | 1 | |a Mertens, Pascal |e verfasserin |4 aut | |
| 700 | 1 | |a Quispe-Tintaya, Wilber |e verfasserin |4 aut | |
| 700 | 1 | |a Deborggraeve, Stijn |e verfasserin |4 aut | |
| 700 | 1 | |a De Doncker, Simonne |e verfasserin |4 aut | |
| 700 | 1 | |a Leclipteux, Thierry |e verfasserin |4 aut | |
| 700 | 1 | |a Bañuls, Anne-Laure |e verfasserin |4 aut | |
| 700 | 1 | |a Büscher, Philippe |e verfasserin |4 aut | |
| 700 | 1 | |a Dujardin, Jean-Claude |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Diagnostic microbiology and infectious disease |d 1986 |g 63(2009), 2 vom: 12. Feb., Seite 173-81 |w (DE-627)NLM01262845X |x 1879-0070 |7 nnns |
| 773 | 1 | 8 | |g volume:63 |g year:2009 |g number:2 |g day:12 |g month:02 |g pages:173-81 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.diagmicrobio.2008.10.015 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 63 |j 2009 |e 2 |b 12 |c 02 |h 173-81 | ||