Preparative isolation of pseudolaric acids A and B, and their glucosides from the root bark of Pseudolarix kaempferi using high-speed counter-current chromatography
In order to provide the chemical markers for the quality control of herbal medicines, four diterpenoids, pseudolaric acids A and B (PAA and PAB), and their glucosides were isolated from the methanol extract of the Chinese herb Pseudolarix kaempferi using high-speed counter-current chromatography (HSCCC). The diphase solvent system was n-hexane/EtOAc/MeOH/H(2)O which was used at two ratios (5:5:5:5 and 1:9:4:6 by volume) in the separation of pseudolaric acids and their glycosides, respectively. As a result, PAA (14 mg), PAB (129 mg), PAA-O-beta-D-glucopyranoside (8 mg, PAAG), and PAB-O-beta-D-glucopyranoside (42 mg, PABG) were obtained from 0.5 g of the crude extract. Their purities were determined to be above 97% by HPLC analysis. Their chemical structures were confirmed by( 1)H and( 13)C NMR analysis or HPLC comparison with the reference compounds.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2009 |
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| Erschienen: |
2009 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:32 |
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| Enthalten in: |
Journal of separation science - 32(2009), 2 vom: 15. Jan., Seite 309-13 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Han, Quan-Bin [VerfasserIn] |
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| Links: |
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| Themen: |
82508-31-4 |
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| Anmerkungen: |
Date Completed 20.04.2009 Date Revised 02.02.2009 published: Print Citation Status MEDLINE |
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| doi: |
10.1002/jssc.200800547 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM185085210 |
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| 100 | 1 | |a Han, Quan-Bin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Preparative isolation of pseudolaric acids A and B, and their glucosides from the root bark of Pseudolarix kaempferi using high-speed counter-current chromatography |
| 264 | 1 | |c 2009 | |
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| 500 | |a Date Completed 20.04.2009 | ||
| 500 | |a Date Revised 02.02.2009 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a In order to provide the chemical markers for the quality control of herbal medicines, four diterpenoids, pseudolaric acids A and B (PAA and PAB), and their glucosides were isolated from the methanol extract of the Chinese herb Pseudolarix kaempferi using high-speed counter-current chromatography (HSCCC). The diphase solvent system was n-hexane/EtOAc/MeOH/H(2)O which was used at two ratios (5:5:5:5 and 1:9:4:6 by volume) in the separation of pseudolaric acids and their glycosides, respectively. As a result, PAA (14 mg), PAB (129 mg), PAA-O-beta-D-glucopyranoside (8 mg, PAAG), and PAB-O-beta-D-glucopyranoside (42 mg, PABG) were obtained from 0.5 g of the crude extract. Their purities were determined to be above 97% by HPLC analysis. Their chemical structures were confirmed by( 1)H and( 13)C NMR analysis or HPLC comparison with the reference compounds | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 650 | 7 | |a Glucosides |2 NLM | |
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| 700 | 1 | |a Wong, Lina |e verfasserin |4 aut | |
| 700 | 1 | |a Lai, Fanny |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Nian-Yun |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Jing-Zheng |e verfasserin |4 aut | |
| 700 | 1 | |a Qiao, Chun-Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Hong-Xi |e verfasserin |4 aut | |
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