The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins
Biochemical assay of proteomic libraries derived from the Saccharomyces cerevisiae genome provides a powerful new tool for the assignment of activities to proteins. Particular advantages of this approach include the speed with which a protein can be identified and the generality for any biological activity for which an assay can be developed. We discuss the utility of this approach for the identification of RNA-modifying enzymes using a yeast proteomic library derived from a genomic set of strains expressing GST-ORF fusion proteins. This technique is also broadly applicable to other classes of RNA-protein interactions, including RNA binding and RNA degradation, and can be used with any of the proteomic libraries that are available.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2008 |
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| Erschienen: |
2008 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:488 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 488(2008) vom: 22., Seite 383-93 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jackman, Jane E [VerfasserIn] |
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| Links: |
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| Themen: |
63231-63-0 |
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| Anmerkungen: |
Date Completed 12.01.2009 Date Revised 13.11.2018 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-60327-475-3_25 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM184264944 |
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| 245 | 1 | 4 | |a The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins |
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| 520 | |a Biochemical assay of proteomic libraries derived from the Saccharomyces cerevisiae genome provides a powerful new tool for the assignment of activities to proteins. Particular advantages of this approach include the speed with which a protein can be identified and the generality for any biological activity for which an assay can be developed. We discuss the utility of this approach for the identification of RNA-modifying enzymes using a yeast proteomic library derived from a genomic set of strains expressing GST-ORF fusion proteins. This technique is also broadly applicable to other classes of RNA-protein interactions, including RNA binding and RNA degradation, and can be used with any of the proteomic libraries that are available | ||
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| 700 | 1 | |a Phizicky, Eric M |e verfasserin |4 aut | |
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