HBV infection downregulated Mre11 expression and induced genome instability
OBJECTIVE: Prolonged infection with Hepatitis B virus (HBV) has been recognized as a major factor for hepatocellular carcinoma (HCC). In this study, we studied the host protein Mrel11 fluctuations after HBV infection which might be eventually contributed to cell transformation.
METHODS: Western Blot was monitored to detect the expression of Mre11, and RNA interference was used to downregulate protein expression. Then, Ligation mediated PCR was used to detect the level of DNA double strand breaks.
RESULTS: Mre11 protein was downregulated when HBV infection occured, and the downregulated expression was also seen in HCC tissue. By RNA interference, we found that Mre11 knockdown caused DNA instability.
CONCLUSION: Mre11 expression downregulation contributed at least partially to cell transformation caused by HBV infection.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2008 |
|---|---|
| Erschienen: |
2008 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:48 |
|---|---|
| Enthalten in: |
Wei sheng wu xue bao = Acta microbiologica Sinica - 48(2008), 8 vom: 01. Aug., Seite 1031-4 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Liu, Yu [VerfasserIn] |
|---|
| Themen: |
DNA-Binding Proteins |
|---|
| Anmerkungen: |
Date Completed 01.06.2009 Date Revised 28.08.2019 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM184018005 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM184018005 | ||
| 003 | DE-627 | ||
| 005 | 20231223165801.0 | ||
| 007 | tu | ||
| 008 | 231223s2008 xx ||||| 00| ||chi c | ||
| 028 | 5 | 2 | |a pubmed24n0613.xml |
| 035 | |a (DE-627)NLM184018005 | ||
| 035 | |a (NLM)18956751 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a chi | ||
| 100 | 1 | |a Liu, Yu |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a HBV infection downregulated Mre11 expression and induced genome instability |
| 264 | 1 | |c 2008 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 01.06.2009 | ||
| 500 | |a Date Revised 28.08.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: Prolonged infection with Hepatitis B virus (HBV) has been recognized as a major factor for hepatocellular carcinoma (HCC). In this study, we studied the host protein Mrel11 fluctuations after HBV infection which might be eventually contributed to cell transformation | ||
| 520 | |a METHODS: Western Blot was monitored to detect the expression of Mre11, and RNA interference was used to downregulate protein expression. Then, Ligation mediated PCR was used to detect the level of DNA double strand breaks | ||
| 520 | |a RESULTS: Mre11 protein was downregulated when HBV infection occured, and the downregulated expression was also seen in HCC tissue. By RNA interference, we found that Mre11 knockdown caused DNA instability | ||
| 520 | |a CONCLUSION: Mre11 expression downregulation contributed at least partially to cell transformation caused by HBV infection | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA-Binding Proteins |2 NLM | |
| 650 | 7 | |a MRE11 protein, human |2 NLM | |
| 650 | 7 | |a MRE11 Homologue Protein |2 NLM | |
| 650 | 7 | |a EC 3.1.- |2 NLM | |
| 700 | 1 | |a Hou, Ningbo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Fan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yanhong |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a He, Xiang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong, Hui |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Wei sheng wu xue bao = Acta microbiologica Sinica |d 1985 |g 48(2008), 8 vom: 01. Aug., Seite 1031-4 |w (DE-627)NLM012631655 |x 0001-6209 |7 nnns |
| 773 | 1 | 8 | |g volume:48 |g year:2008 |g number:8 |g day:01 |g month:08 |g pages:1031-4 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 48 |j 2008 |e 8 |b 01 |c 08 |h 1031-4 | ||