RNAIII-independent target gene control by the agr quorum-sensing system : insight into the evolution of virulence regulation in Staphylococcus aureus
Cell-density-dependent gene regulation by quorum-sensing systems has a crucial function in bacterial physiology and pathogenesis. We demonstrate here that the Staphylococcus aureus agr quorum-sensing regulon is divided into (1) control of metabolism and PSM cytolysin genes, which occurs independently of the small regulatory RNA RNAIII, and (2) RNAIII-dependent control of additional virulence genes. Remarkably, PSM expression was regulated by direct binding of the AgrA response regulator. Our findings suggest that quorum-sensing regulation of PSMs was established before wide-ranging control of virulence was added to the agr regulon, which likely occurred by development of the RNAIII-encoding region around the gene encoding the PSM delta-toxin. Moreover, the agr regulon in the community-associated methicillin-resistant S. aureus MW2 considerably differed from that previously determined using laboratory strains. By establishing a two-level model of quorum-sensing target gene regulation in S. aureus, our study gives important insight into the evolution of virulence control in this leading human pathogen.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2008 |
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| Erschienen: |
2008 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:32 |
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| Enthalten in: |
Molecular cell - 32(2008), 1 vom: 10. Okt., Seite 150-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Queck, Shu Y [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 12.11.2008 Date Revised 03.10.2019 published: Print Citation Status MEDLINE |
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| doi: |
10.1016/j.molcel.2008.08.005 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM182991040 |
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| 520 | |a Cell-density-dependent gene regulation by quorum-sensing systems has a crucial function in bacterial physiology and pathogenesis. We demonstrate here that the Staphylococcus aureus agr quorum-sensing regulon is divided into (1) control of metabolism and PSM cytolysin genes, which occurs independently of the small regulatory RNA RNAIII, and (2) RNAIII-dependent control of additional virulence genes. Remarkably, PSM expression was regulated by direct binding of the AgrA response regulator. Our findings suggest that quorum-sensing regulation of PSMs was established before wide-ranging control of virulence was added to the agr regulon, which likely occurred by development of the RNAIII-encoding region around the gene encoding the PSM delta-toxin. Moreover, the agr regulon in the community-associated methicillin-resistant S. aureus MW2 considerably differed from that previously determined using laboratory strains. By establishing a two-level model of quorum-sensing target gene regulation in S. aureus, our study gives important insight into the evolution of virulence control in this leading human pathogen | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Intramural | |
| 650 | 7 | |a Agr protein, Staphylococcus aureus |2 NLM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Bacterial Toxins |2 NLM | |
| 650 | 7 | |a DNA, Bacterial |2 NLM | |
| 650 | 7 | |a RNA, Bacterial |2 NLM | |
| 650 | 7 | |a RNAIII, Staphylococcus aureus |2 NLM | |
| 650 | 7 | |a Trans-Activators |2 NLM | |
| 700 | 1 | |a Jameson-Lee, Max |e verfasserin |4 aut | |
| 700 | 1 | |a Villaruz, Amer E |e verfasserin |4 aut | |
| 700 | 1 | |a Bach, Thanh-Huy L |e verfasserin |4 aut | |
| 700 | 1 | |a Khan, Burhan A |e verfasserin |4 aut | |
| 700 | 1 | |a Sturdevant, Daniel E |e verfasserin |4 aut | |
| 700 | 1 | |a Ricklefs, Stacey M |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Otto, Michael |e verfasserin |4 aut | |
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