Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-alpha release of endothelial cells
OBJECTIVE: To explore the effects of down-regulated tryptase expression in mast cells on the synthesis and release of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) of vascular endothelial cells.
METHODS: Tryptase-siRNA (small-interfering RNA) vector was constructed to inhibit tryptase expression in P815 cells. The medium of P815 cells treated by the tryptase-siRNA (RNAi-P815 group) or pure vector (P815 group) was collected and used to culture bEnd.3 cells. The messenger RNAs (mRNAs) of IL-6 and TNF-alpha in bEnd.3 cells and their protein levels in the medium were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively.
RESULTS: IL-6 and TNF-alpha mRNAs in bEnd.3 cells cultured in RNAi-P815-conditioned medium decreased significantly compared to those in P815-conditioned medium. Consistently, IL-6 and TNF-alpha protein levels in the medium of bEnd.3 of RNAi-P815 group were lower than those of P815 group.
CONCLUSION: Reduced tryptase expression significantly inhibited the synthesis and release of IL-6 and TNF-alpha in vascular endothelial cells. RNA interference targeting tryptase expression may be a new anti-inflammatory strategy for vascular diseases.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2008 |
|---|---|
| Erschienen: |
2008 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
|---|---|
| Enthalten in: |
Journal of Zhejiang University. Science. B - 9(2008), 8 vom: 28. Aug., Seite 656-61 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Jiang, Yi-feng [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Culture Media, Conditioned |
|---|
| Anmerkungen: |
Date Completed 19.02.2009 Date Revised 20.10.2021 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1631/jzus.B0810188 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM182159531 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM182159531 | ||
| 003 | DE-627 | ||
| 005 | 20231223162427.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231223s2008 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1631/jzus.B0810188 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0607.xml |
| 035 | |a (DE-627)NLM182159531 | ||
| 035 | |a (NLM)18763316 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Jiang, Yi-feng |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-alpha release of endothelial cells |
| 264 | 1 | |c 2008 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 19.02.2009 | ||
| 500 | |a Date Revised 20.10.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To explore the effects of down-regulated tryptase expression in mast cells on the synthesis and release of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) of vascular endothelial cells | ||
| 520 | |a METHODS: Tryptase-siRNA (small-interfering RNA) vector was constructed to inhibit tryptase expression in P815 cells. The medium of P815 cells treated by the tryptase-siRNA (RNAi-P815 group) or pure vector (P815 group) was collected and used to culture bEnd.3 cells. The messenger RNAs (mRNAs) of IL-6 and TNF-alpha in bEnd.3 cells and their protein levels in the medium were measured by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively | ||
| 520 | |a RESULTS: IL-6 and TNF-alpha mRNAs in bEnd.3 cells cultured in RNAi-P815-conditioned medium decreased significantly compared to those in P815-conditioned medium. Consistently, IL-6 and TNF-alpha protein levels in the medium of bEnd.3 of RNAi-P815 group were lower than those of P815 group | ||
| 520 | |a CONCLUSION: Reduced tryptase expression significantly inhibited the synthesis and release of IL-6 and TNF-alpha in vascular endothelial cells. RNA interference targeting tryptase expression may be a new anti-inflammatory strategy for vascular diseases | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Culture Media, Conditioned |2 NLM | |
| 650 | 7 | |a Interleukin-6 |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a RNA, Small Interfering |2 NLM | |
| 650 | 7 | |a Tumor Necrosis Factor-alpha |2 NLM | |
| 650 | 7 | |a Tryptases |2 NLM | |
| 650 | 7 | |a EC 3.4.21.59 |2 NLM | |
| 700 | 1 | |a Zhao, Feng-di |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xiao-bo |e verfasserin |4 aut | |
| 700 | 1 | |a Ning, Yan-xia |e verfasserin |4 aut | |
| 700 | 1 | |a Zhi, Xiu-ling |e verfasserin |4 aut | |
| 700 | 1 | |a Qian, Rui-zhe |e verfasserin |4 aut | |
| 700 | 1 | |a Yin, Lian-hua |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of Zhejiang University. Science. B |d 2005 |g 9(2008), 8 vom: 28. Aug., Seite 656-61 |w (DE-627)NLM152588027 |x 1862-1783 |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2008 |g number:8 |g day:28 |g month:08 |g pages:656-61 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1631/jzus.B0810188 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 9 |j 2008 |e 8 |b 28 |c 08 |h 656-61 | ||