A novel protein microarray detection technique based on biotin-avidin conjugation probe
In this experiment, a novel biotin-avidin conjugation probe was synthesized and employed in the detection of reverse-phase protein microarray. Firstly, the proportion of the biotin-avidin conjugation probe was optimized. Then the rat IgG and goat anti-rat IgG system was served as a model to optimize the fabrication conditions of reverse-phase protein microarray, including the non-specific absorption of streptavidin-Cy3 molecules, spotting buffer as well as protein activities. At last, the biotin-avidin conjugation probe was applied to the detection of the reverse-phase protein microarray. The results show that the protein microarray prepared by using BSA spotting buffer could prevent non-specific absorptions of fluorescent molecules and improve the sensitivity, effectively. In addition, compared with traditional biotin-avidin system, the detection limit could be improved four times using the biotin-avidin conjugation probe. In conclusion, the biotin-avidin conjugation probe has its merits of easy synthesis, low price and could be further conjugated with other signal amplification techniques, which is promising to be used in the detection of protein microarray.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2008 |
|---|---|
| Erschienen: |
2008 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
|---|---|
| Enthalten in: |
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 24(2008), 3 vom: 15. März, Seite 515-20 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Yu, Xiaobo [VerfasserIn] |
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| Themen: |
1405-69-2 |
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| Anmerkungen: |
Date Completed 08.09.2009 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM180552260 |
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| 100 | 1 | |a Yu, Xiaobo |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A novel protein microarray detection technique based on biotin-avidin conjugation probe |
| 264 | 1 | |c 2008 | |
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| 500 | |a Date Completed 08.09.2009 | ||
| 500 | |a Date Revised 21.11.2013 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a In this experiment, a novel biotin-avidin conjugation probe was synthesized and employed in the detection of reverse-phase protein microarray. Firstly, the proportion of the biotin-avidin conjugation probe was optimized. Then the rat IgG and goat anti-rat IgG system was served as a model to optimize the fabrication conditions of reverse-phase protein microarray, including the non-specific absorption of streptavidin-Cy3 molecules, spotting buffer as well as protein activities. At last, the biotin-avidin conjugation probe was applied to the detection of the reverse-phase protein microarray. The results show that the protein microarray prepared by using BSA spotting buffer could prevent non-specific absorptions of fluorescent molecules and improve the sensitivity, effectively. In addition, compared with traditional biotin-avidin system, the detection limit could be improved four times using the biotin-avidin conjugation probe. In conclusion, the biotin-avidin conjugation probe has its merits of easy synthesis, low price and could be further conjugated with other signal amplification techniques, which is promising to be used in the detection of protein microarray | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA Probes |2 NLM | |
| 650 | 7 | |a Immunoglobulin G |2 NLM | |
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| 700 | 1 | |a Zhao, Tianming |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Zhidan |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Hongkun |e verfasserin |4 aut | |
| 700 | 1 | |a He, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Danke |e verfasserin |4 aut | |
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