A nonribosomal peptide synthetase gene tzw1 is involved in zwittermicin A biosynthesis in Bacillus thuringiensis G03
A 4.20-kb SspI fragment from Bacillus thuringiensis G03 was cloned and sequenced. Sequencing analysis revealed two complete open reading frames (ORF; tzw1 and tzw2), and one incomplete ORF (tzw3) (GenBank accession no. EU293887). Tzw1 encodes a putative nonribosomal peptide synthetase with thiolation and condensation domains localized at the C-termini, whereas tzw2 and tzw3 encode acyl carrier protein and Acyl-CoA dehydrogenase, respectively. To investigate the function of tzw1 in zwittermicin A (ZA) biosynthesis, an in-frame deletion of 1,461 bp within tzw1 was constructed. The mutant abolished ZA production. Complementation of the mutant with cloned tzw1 restored ZA productivity. These results revealed that tzw1 is required for ZA biosynthesis in B. thuringiensis G03.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2008 |
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| Erschienen: |
2008 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:57 |
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| Enthalten in: |
Current microbiology - 57(2008), 1 vom: 14. Juli, Seite 61-5 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shao, Tiemei [VerfasserIn] |
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| Links: |
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| Themen: |
155547-95-8 |
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| Anmerkungen: |
Date Completed 11.08.2008 Date Revised 20.10.2021 published: Print-Electronic GENBANK: EU293887 Citation Status MEDLINE |
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| doi: |
10.1007/s00284-008-9153-5 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM17925152X |
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| 100 | 1 | |a Shao, Tiemei |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A nonribosomal peptide synthetase gene tzw1 is involved in zwittermicin A biosynthesis in Bacillus thuringiensis G03 |
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| 500 | |a Date Completed 11.08.2008 | ||
| 500 | |a Date Revised 20.10.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a GENBANK: EU293887 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A 4.20-kb SspI fragment from Bacillus thuringiensis G03 was cloned and sequenced. Sequencing analysis revealed two complete open reading frames (ORF; tzw1 and tzw2), and one incomplete ORF (tzw3) (GenBank accession no. EU293887). Tzw1 encodes a putative nonribosomal peptide synthetase with thiolation and condensation domains localized at the C-termini, whereas tzw2 and tzw3 encode acyl carrier protein and Acyl-CoA dehydrogenase, respectively. To investigate the function of tzw1 in zwittermicin A (ZA) biosynthesis, an in-frame deletion of 1,461 bp within tzw1 was constructed. The mutant abolished ZA production. Complementation of the mutant with cloned tzw1 restored ZA productivity. These results revealed that tzw1 is required for ZA biosynthesis in B. thuringiensis G03 | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 7 | |a Bacterial Proteins |2 NLM | |
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| 700 | 1 | |a Bai, Linquan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Guangjun |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Daqun |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Zhuofu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jianmi |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Fuping |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Dafang |e verfasserin |4 aut | |
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