An acute model for atrial fibrillation arising from a peripheral atrial site : evidence for primary and secondary triggers
BACKGROUND: We previously demonstrated that acetylcholine (Ach) injected into cardiac ganglionated plexi (GP) causes pulmonary vein (PV) ectopy initiating atrial fibrillation (AF).
OBJECTIVE: To determine the effects of Ach applied at non-PV sites.
METHODS: Overall, 54 dogs were anesthetized with Na-pentobarbital. A right and left thoracotomy allowed the placement of multielectrode catheters to record from the superior PVs, mid portion of the atrium and the atrial appendages (AA). A monophasic action potential (MAP) was recorded from the AA. Ach (1, 10, 100 mM) was applied sequentially to the AA.
RESULTS: In 19 of 26 animals, Ach 100 mM on the right (n = 15) or left (n = 4) AA induced focal, sustained AF (>or=10 minutes) with rapid regular firing (cycle length = 37 +/- 7 ms) at the AA. A clamp with teeth placed across the AA caused arrest in the AA. However, AF was sustained only when PV sites adjacent to the GP manifested complex fractionated atrial electrograms (CFAE). Clamping the AA prior to Ach (100 mM) application resulted in focal AF arising at the PVs but not at the AA. When a clamp without teeth was applied prior to Ach application, no AF at either AA or PV site could be induced.
CONCLUSION: Isolation of the focal AF at the AA (primary trigger) by clamping caused cessation of activity in the AA, but AF continued due to secondary triggers arising from PVs. The possible mechanism(s) responsible for these findings are discussed, and various ancillary experiments (n = 28) were added to help elucidate mechanisms.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2008 |
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| Erschienen: |
2008 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
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| Enthalten in: |
Journal of cardiovascular electrophysiology - 19(2008), 5 vom: 15. Mai, Seite 519-27 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Scherlag, Benjamin J [VerfasserIn] |
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| Links: |
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| Themen: |
Acetylcholine |
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| Anmerkungen: |
Date Completed 04.06.2008 Date Revised 21.11.2013 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/j.1540-8167.2007.01087.x |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM177540273 |
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| 008 | 231223s2008 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/j.1540-8167.2007.01087.x |2 doi | |
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| 041 | |a eng | ||
| 100 | 1 | |a Scherlag, Benjamin J |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An acute model for atrial fibrillation arising from a peripheral atrial site |b evidence for primary and secondary triggers |
| 264 | 1 | |c 2008 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 04.06.2008 | ||
| 500 | |a Date Revised 21.11.2013 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: We previously demonstrated that acetylcholine (Ach) injected into cardiac ganglionated plexi (GP) causes pulmonary vein (PV) ectopy initiating atrial fibrillation (AF) | ||
| 520 | |a OBJECTIVE: To determine the effects of Ach applied at non-PV sites | ||
| 520 | |a METHODS: Overall, 54 dogs were anesthetized with Na-pentobarbital. A right and left thoracotomy allowed the placement of multielectrode catheters to record from the superior PVs, mid portion of the atrium and the atrial appendages (AA). A monophasic action potential (MAP) was recorded from the AA. Ach (1, 10, 100 mM) was applied sequentially to the AA | ||
| 520 | |a RESULTS: In 19 of 26 animals, Ach 100 mM on the right (n = 15) or left (n = 4) AA induced focal, sustained AF (>or=10 minutes) with rapid regular firing (cycle length = 37 +/- 7 ms) at the AA. A clamp with teeth placed across the AA caused arrest in the AA. However, AF was sustained only when PV sites adjacent to the GP manifested complex fractionated atrial electrograms (CFAE). Clamping the AA prior to Ach (100 mM) application resulted in focal AF arising at the PVs but not at the AA. When a clamp without teeth was applied prior to Ach application, no AF at either AA or PV site could be induced | ||
| 520 | |a CONCLUSION: Isolation of the focal AF at the AA (primary trigger) by clamping caused cessation of activity in the AA, but AF continued due to secondary triggers arising from PVs. The possible mechanism(s) responsible for these findings are discussed, and various ancillary experiments (n = 28) were added to help elucidate mechanisms | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Acetylcholine |2 NLM | |
| 650 | 7 | |a N9YNS0M02X |2 NLM | |
| 700 | 1 | |a Hou, Ying-Long |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Jiaxiong |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Zhibing |e verfasserin |4 aut | |
| 700 | 1 | |a Zacharias, Soni |e verfasserin |4 aut | |
| 700 | 1 | |a Dasari, Tarun |e verfasserin |4 aut | |
| 700 | 1 | |a Niu, Guodong |e verfasserin |4 aut | |
| 700 | 1 | |a Ghias, Muhammad |e verfasserin |4 aut | |
| 700 | 1 | |a Patterson, Eugene |e verfasserin |4 aut | |
| 700 | 1 | |a Jackman, Warren M |e verfasserin |4 aut | |
| 700 | 1 | |a Lazzara, Ralph |e verfasserin |4 aut | |
| 700 | 1 | |a Po, Sunny S |e verfasserin |4 aut | |
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