TAp63gamma-induced apoptosis mediated by apoptosis inducing factor in human esophageal squamous carcinoma EC9706 cells
OBJECTIVE: To study the molecular mechanism of TAp63gamma-induced cell apoptosis.
METHODS: Transcription and protein expression of apoptosis inducing factor and p63 were investigated by immunohistochemistry and RT-PCR in human esophageal squamous carcinoma cell line EC9706 respectively. Twenty-four hours after transfection with pcDNA3.1-TAp63gamma, the apoptosis and translocation of apoptosis inducing factor in EC9706 cells were studied by flow cytometry, laser confocal microscopy and mitochondrial/cytosol/nuclear extraction analysis respectively. Down-regulation of apoptosis inducing factor protein was achieved by RNAi and pretreatment with caspase inhibitor zVAD.fmk of EC9706 cells.
RESULTS: Presence of protein expressions of apoptosis inducing factor and absence of TAp63gamma was observed in the cytoplasm of untransfected cells. RT-PCR verified the subtype of p63 in EC9706 cells was DeltaNp63. After 24 hours of transfection, both nuclear and cytoplasmic expression of apoptosis inducing factor protein were observed in cells transfected with TAp63gamma and p53 expression vectors, but not in cells transfected with control vector. Cell apoptosis rates were 1.37%, 13.64%, 4.52%, 4.03% and 1.91% in the pcDNA3.1 transfection group, pcDNA3.1-TAp63gamma transfection group, apoptosis inducing factor siRNA and pcDNA3.1-TAp63gamma transfection group, zVAD.fmk treatment group, and the group receiving apoptosis inducing factor siRNA, plus zVAD.fmk treatment and pcDNA3.1-TAp63gamma transfection, respectively.
CONCLUSIONS: Apoptosis inducing factor of EC9706 cells is released from mitochondria into both the cytoplasm and nucleus during TAp63gamma induced apoptosis. Down-regulation of apoptosis inducing factor inhibits TAp63gamma-induced apoptosis. Overall, TAp63gamma-induced apoptosis is dependent on the expression of apoptosis inducing factor and caspase.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2007 |
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| Erschienen: |
2007 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
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| Enthalten in: |
Zhonghua bing li xue za zhi = Chinese journal of pathology - 36(2007), 6 vom: 07. Juni, Seite 384-9 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Fan, Tian-li [VerfasserIn] |
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| Anmerkungen: |
Date Completed 01.07.2010 Date Revised 18.08.2016 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM173328482 |
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| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM173328482 | ||
| 003 | DE-627 | ||
| 005 | 20231223133554.0 | ||
| 007 | tu | ||
| 008 | 231223s2007 xx ||||| 00| ||chi c | ||
| 028 | 5 | 2 | |a pubmed24n0578.xml |
| 035 | |a (DE-627)NLM173328482 | ||
| 035 | |a (NLM)17822623 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a chi | ||
| 100 | 1 | |a Fan, Tian-li |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a TAp63gamma-induced apoptosis mediated by apoptosis inducing factor in human esophageal squamous carcinoma EC9706 cells |
| 264 | 1 | |c 2007 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 01.07.2010 | ||
| 500 | |a Date Revised 18.08.2016 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To study the molecular mechanism of TAp63gamma-induced cell apoptosis | ||
| 520 | |a METHODS: Transcription and protein expression of apoptosis inducing factor and p63 were investigated by immunohistochemistry and RT-PCR in human esophageal squamous carcinoma cell line EC9706 respectively. Twenty-four hours after transfection with pcDNA3.1-TAp63gamma, the apoptosis and translocation of apoptosis inducing factor in EC9706 cells were studied by flow cytometry, laser confocal microscopy and mitochondrial/cytosol/nuclear extraction analysis respectively. Down-regulation of apoptosis inducing factor protein was achieved by RNAi and pretreatment with caspase inhibitor zVAD.fmk of EC9706 cells | ||
| 520 | |a RESULTS: Presence of protein expressions of apoptosis inducing factor and absence of TAp63gamma was observed in the cytoplasm of untransfected cells. RT-PCR verified the subtype of p63 in EC9706 cells was DeltaNp63. After 24 hours of transfection, both nuclear and cytoplasmic expression of apoptosis inducing factor protein were observed in cells transfected with TAp63gamma and p53 expression vectors, but not in cells transfected with control vector. Cell apoptosis rates were 1.37%, 13.64%, 4.52%, 4.03% and 1.91% in the pcDNA3.1 transfection group, pcDNA3.1-TAp63gamma transfection group, apoptosis inducing factor siRNA and pcDNA3.1-TAp63gamma transfection group, zVAD.fmk treatment group, and the group receiving apoptosis inducing factor siRNA, plus zVAD.fmk treatment and pcDNA3.1-TAp63gamma transfection, respectively | ||
| 520 | |a CONCLUSIONS: Apoptosis inducing factor of EC9706 cells is released from mitochondria into both the cytoplasm and nucleus during TAp63gamma induced apoptosis. Down-regulation of apoptosis inducing factor inhibits TAp63gamma-induced apoptosis. Overall, TAp63gamma-induced apoptosis is dependent on the expression of apoptosis inducing factor and caspase | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Amino Acid Chloromethyl Ketones |2 NLM | |
| 650 | 7 | |a Apoptosis Inducing Factor |2 NLM | |
| 650 | 7 | |a Caspase Inhibitors |2 NLM | |
| 650 | 7 | |a RNA, Small Interfering |2 NLM | |
| 650 | 7 | |a TP63 protein, human |2 NLM | |
| 650 | 7 | |a Trans-Activators |2 NLM | |
| 650 | 7 | |a Transcription Factors |2 NLM | |
| 650 | 7 | |a Tumor Suppressor Proteins |2 NLM | |
| 650 | 7 | |a benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone |2 NLM | |
| 700 | 1 | |a Hao, Yi-bin |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Pei-rong |e verfasserin |4 aut | |
| 700 | 1 | |a Hou, Gui-qin |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Guo-zhong |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Guan-rui |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Zhonghua bing li xue za zhi = Chinese journal of pathology |d 1985 |g 36(2007), 6 vom: 07. Juni, Seite 384-9 |w (DE-627)NLM012624403 |x 0529-5807 |7 nnns |
| 773 | 1 | 8 | |g volume:36 |g year:2007 |g number:6 |g day:07 |g month:06 |g pages:384-9 |
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| 952 | |d 36 |j 2007 |e 6 |b 07 |c 06 |h 384-9 | ||