Thimerosal-induced apoptosis in human SCM1 gastric cancer cells : activation of p38 MAP kinase and caspase-3 pathways without involvement of [Ca2+]i elevation
Thimerosal is a mercury-containing preservative in some vaccines. The effect of thimerosal on human gastric cancer cells is unknown. This study shows that in cultured human gastric cancer cells (SCM1), thimerosal reduced cell viability in a concentration- and time-dependent manner. Thimerosal caused apoptosis as assessed by propidium iodide-stained cells and caspase-3 activation. Although immunoblotting data revealed that thimerosal could activate the phosphorylation of extracellular signal-regulated kinase, c-Jun NH2-terminal protein kinase, and p38 mitogen-activated protein kinase (p38 MAPK), only SB203580 (a p38 MAPK inhibitor) partially prevented cells from apoptosis. Thimerosal also induced [Ca2+](i) increases via Ca2+ influx from the extracellular space. However, pretreatment with (bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetate)/AM, a Ca2+ chelator, to prevent thimerosal-induced [Ca2+](i) increases did not protect cells from death. The results suggest that in SCM1 cells, thimerosal caused Ca2+-independent apoptosis via phosphorylating p38 MAPK resulting in caspase-3 activation.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2007 |
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| Erschienen: |
2007 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:100 |
|---|---|
| Enthalten in: |
Toxicological sciences : an official journal of the Society of Toxicology - 100(2007), 1 vom: 01. Nov., Seite 109-17 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Shiuh-Inn [VerfasserIn] |
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| Anmerkungen: |
Date Completed 06.12.2007 Date Revised 15.08.2018 published: Print-Electronic Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM172117054 |
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| 041 | |a eng | ||
| 100 | 1 | |a Liu, Shiuh-Inn |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Thimerosal-induced apoptosis in human SCM1 gastric cancer cells |b activation of p38 MAP kinase and caspase-3 pathways without involvement of [Ca2+]i elevation |
| 264 | 1 | |c 2007 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
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| 500 | |a Date Completed 06.12.2007 | ||
| 500 | |a Date Revised 15.08.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Thimerosal is a mercury-containing preservative in some vaccines. The effect of thimerosal on human gastric cancer cells is unknown. This study shows that in cultured human gastric cancer cells (SCM1), thimerosal reduced cell viability in a concentration- and time-dependent manner. Thimerosal caused apoptosis as assessed by propidium iodide-stained cells and caspase-3 activation. Although immunoblotting data revealed that thimerosal could activate the phosphorylation of extracellular signal-regulated kinase, c-Jun NH2-terminal protein kinase, and p38 mitogen-activated protein kinase (p38 MAPK), only SB203580 (a p38 MAPK inhibitor) partially prevented cells from apoptosis. Thimerosal also induced [Ca2+](i) increases via Ca2+ influx from the extracellular space. However, pretreatment with (bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetate)/AM, a Ca2+ chelator, to prevent thimerosal-induced [Ca2+](i) increases did not protect cells from death. The results suggest that in SCM1 cells, thimerosal caused Ca2+-independent apoptosis via phosphorylating p38 MAPK resulting in caspase-3 activation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Anthracenes |2 NLM | |
| 650 | 7 | |a Chelating Agents |2 NLM | |
| 650 | 7 | |a Flavonoids |2 NLM | |
| 650 | 7 | |a Imidazoles |2 NLM | |
| 650 | 7 | |a Preservatives, Pharmaceutical |2 NLM | |
| 650 | 7 | |a Protein Kinase Inhibitors |2 NLM | |
| 650 | 7 | |a Pyridines |2 NLM | |
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| 650 | 7 | |a pyrazolanthrone |2 NLM | |
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| 650 | 7 | |a Thimerosal |2 NLM | |
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| 650 | 7 | |a Egtazic Acid |2 NLM | |
| 650 | 7 | |a 526U7A2651 |2 NLM | |
| 650 | 7 | |a Extracellular Signal-Regulated MAP Kinases |2 NLM | |
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| 650 | 7 | |a SB 203580 |2 NLM | |
| 650 | 7 | |a OU13V1EYWQ |2 NLM | |
| 650 | 7 | |a 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one |2 NLM | |
| 650 | 7 | |a SJE1IO5E3I |2 NLM | |
| 700 | 1 | |a Huang, Chorng-Chih |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Chun-Jen |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Being-Whey |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Po-Min |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Yi-Chien |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Wei-Chuan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jue-Long |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Yih-Chau |e verfasserin |4 aut | |
| 700 | 1 | |a Chu, Sau-Tung |e verfasserin |4 aut | |
| 700 | 1 | |a Chou, Chiang-Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Jan, Chung-Ren |e verfasserin |4 aut | |
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