Chemical fingerprint and quantitative analysis of fructus psoraleae by high-performance liquid chromatography
Fructus Psoraleae, a widely used traditional Chinese medicine, is well known as a health supplement ingredient. In our study, an improved and comprehensive HPLC fingerprint of Fructus Psoraleae was established. Two important new benzofuran glycosides, psoralenoside and isopsoralenoside, were identified as characteristic constituents for the first time. HPLC separation was performed on an RP-C8 column. The mobile phase was acetonitrile and 0.1% acetic acid solution with linear gradient change of acetonitrile from 10 to 82% in 40 min. The flow rate was 1.0 mL/min, and the detection wavelength was set at 310 nm. The HPLC chromatograms of twenty-six samples from different regions of China showed a similar pattern. Twelve peaks were selected as characteristic peaks and further identified as psoralenoside, isopsoralenoside, psoralen, isopsoralen, bavachromene, corylifolin, corylin, psoralidin, isobavachalcone, bavachinin, corylifol A, and bakuchiol, respectively. Nine of them were simultaneously quantitatively analyzed for the first time. A more comprehensive analytical method was established for the fingerprint of Fructus Psoraleae. It is very useful for authentication and quality assessment of the crude drug.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2007 |
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| Erschienen: |
2007 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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| Enthalten in: |
Journal of separation science - 30(2007), 6 vom: 14. Apr., Seite 813-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Qiao, Chun-Feng [VerfasserIn] |
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| Anmerkungen: |
Date Completed 18.06.2007 Date Revised 25.08.2022 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM170584690 |
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| 041 | |a eng | ||
| 100 | 1 | |a Qiao, Chun-Feng |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Chemical fingerprint and quantitative analysis of fructus psoraleae by high-performance liquid chromatography |
| 264 | 1 | |c 2007 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 18.06.2007 | ||
| 500 | |a Date Revised 25.08.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Fructus Psoraleae, a widely used traditional Chinese medicine, is well known as a health supplement ingredient. In our study, an improved and comprehensive HPLC fingerprint of Fructus Psoraleae was established. Two important new benzofuran glycosides, psoralenoside and isopsoralenoside, were identified as characteristic constituents for the first time. HPLC separation was performed on an RP-C8 column. The mobile phase was acetonitrile and 0.1% acetic acid solution with linear gradient change of acetonitrile from 10 to 82% in 40 min. The flow rate was 1.0 mL/min, and the detection wavelength was set at 310 nm. The HPLC chromatograms of twenty-six samples from different regions of China showed a similar pattern. Twelve peaks were selected as characteristic peaks and further identified as psoralenoside, isopsoralenoside, psoralen, isopsoralen, bavachromene, corylifolin, corylin, psoralidin, isobavachalcone, bavachinin, corylifol A, and bakuchiol, respectively. Nine of them were simultaneously quantitatively analyzed for the first time. A more comprehensive analytical method was established for the fingerprint of Fructus Psoraleae. It is very useful for authentication and quality assessment of the crude drug | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Benzofurans |2 NLM | |
| 650 | 7 | |a Chalcones |2 NLM | |
| 650 | 7 | |a Coumarins |2 NLM | |
| 650 | 7 | |a Drugs, Chinese Herbal |2 NLM | |
| 650 | 7 | |a Flavonoids |2 NLM | |
| 650 | 7 | |a Furocoumarins |2 NLM | |
| 650 | 7 | |a Glycosides |2 NLM | |
| 650 | 7 | |a Phenols |2 NLM | |
| 650 | 7 | |a Solvents |2 NLM | |
| 650 | 7 | |a bavachin |2 NLM | |
| 650 | 7 | |a isopsoralenoside |2 NLM | |
| 650 | 7 | |a psoralenoside |2 NLM | |
| 650 | 7 | |a isobavachalcone |2 NLM | |
| 650 | 7 | |a 20784-50-3 |2 NLM | |
| 650 | 7 | |a angelicin |2 NLM | |
| 650 | 7 | |a CZZ080D7BD |2 NLM | |
| 650 | 7 | |a psoralidin |2 NLM | |
| 650 | 7 | |a G16ZUQ069L |2 NLM | |
| 650 | 7 | |a Ficusin |2 NLM | |
| 650 | 7 | |a KTZ7ZCN2EX |2 NLM | |
| 650 | 7 | |a bakuchiol |2 NLM | |
| 650 | 7 | |a OT12HJU3AR |2 NLM | |
| 700 | 1 | |a Han, Quan-Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Jing-Zheng |e verfasserin |4 aut | |
| 700 | 1 | |a Mo, Shi-Fu |e verfasserin |4 aut | |
| 700 | 1 | |a Kong, Ling-Dong |e verfasserin |4 aut | |
| 700 | 1 | |a Kung, Hsiang-Fu |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Hong-Xi |e verfasserin |4 aut | |
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