Determination of epoxidized soybean oil by gas chromatography/single quadrupole and tandem mass spectrometry stable isotope dilution assay
Copyright (c) 2007 John Wiley & Sons, Ltd..
PVC lids of glass jars often contain epoxidized soybean oil (ESBO), able to migrate and contaminate food. To establish a stable isotope dilution assay (SIDA), the 13C18-labelled internal standard ethyl 9,10,12,13-diepoxyoctadecanoate (13C(18:2E)Et) was synthesized, providing after sample preparation the same retention time as methyl 9,10,12,13-diepoxyoctadecanoate ((18:2E)Me), commonly used as a marker for ESBO in gas chromatographic (GC) analysis. For eleven different food matrices, the GC capillary columns VF-17ms, DB1701 and DB1 were tested with single quadrupole (GC/MS) as well as tandem mass spectrometric detection (GC/MS/MS). Overall, the VF-17ms column coupled with MS/MS detection showed the best results in terms of separation and sensitivity. The method validation for the matrix spiked olive oil resulted in a limit of detection (LOD) of 5 mg kg-1, a limit of quantification (LOQ) of 11 mg kg-1, a mean recovery (n=5, c=106.5 mg kg-1) of 99.7+/-5.5%, with a repeatability (within-run precision) of 6.0%. By means of GC/MS an LOQ of 21 mg kg-1 and a mean recovery (n=5, c=106.5 mg kg-1) of 103.3+/-0.8% with a repeatability of 0.9% were determined.
Medienart: |
Artikel |
---|
Erscheinungsjahr: |
2007 |
---|---|
Erschienen: |
2007 |
Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
---|---|
Enthalten in: |
Rapid communications in mass spectrometry : RCM - 21(2007), 12 vom: 01., Seite 1937-43 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Rothenbacher, Thorsten [VerfasserIn] |
---|
Themen: |
8001-22-7 |
---|
Anmerkungen: |
Date Completed 07.08.2007 Date Revised 31.05.2007 published: Print Citation Status MEDLINE |
---|
Förderinstitution / Projekttitel: |
|
---|
PPN (Katalog-ID): |
NLM170367924 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM170367924 | ||
003 | DE-627 | ||
005 | 20231223123343.0 | ||
007 | tu | ||
008 | 231223s2007 xx ||||| 00| ||eng c | ||
028 | 5 | 2 | |a pubmed24n0568.xml |
035 | |a (DE-627)NLM170367924 | ||
035 | |a (NLM)17510930 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Rothenbacher, Thorsten |e verfasserin |4 aut | |
245 | 1 | 0 | |a Determination of epoxidized soybean oil by gas chromatography/single quadrupole and tandem mass spectrometry stable isotope dilution assay |
264 | 1 | |c 2007 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Completed 07.08.2007 | ||
500 | |a Date Revised 31.05.2007 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright (c) 2007 John Wiley & Sons, Ltd. | ||
520 | |a PVC lids of glass jars often contain epoxidized soybean oil (ESBO), able to migrate and contaminate food. To establish a stable isotope dilution assay (SIDA), the 13C18-labelled internal standard ethyl 9,10,12,13-diepoxyoctadecanoate (13C(18:2E)Et) was synthesized, providing after sample preparation the same retention time as methyl 9,10,12,13-diepoxyoctadecanoate ((18:2E)Me), commonly used as a marker for ESBO in gas chromatographic (GC) analysis. For eleven different food matrices, the GC capillary columns VF-17ms, DB1701 and DB1 were tested with single quadrupole (GC/MS) as well as tandem mass spectrometric detection (GC/MS/MS). Overall, the VF-17ms column coupled with MS/MS detection showed the best results in terms of separation and sensitivity. The method validation for the matrix spiked olive oil resulted in a limit of detection (LOD) of 5 mg kg-1, a limit of quantification (LOQ) of 11 mg kg-1, a mean recovery (n=5, c=106.5 mg kg-1) of 99.7+/-5.5%, with a repeatability (within-run precision) of 6.0%. By means of GC/MS an LOQ of 21 mg kg-1 and a mean recovery (n=5, c=106.5 mg kg-1) of 103.3+/-0.8% with a repeatability of 0.9% were determined | ||
650 | 4 | |a Journal Article | |
650 | 7 | |a Carbon Isotopes |2 NLM | |
650 | 7 | |a Epoxy Compounds |2 NLM | |
650 | 7 | |a Soybean Oil |2 NLM | |
650 | 7 | |a 8001-22-7 |2 NLM | |
700 | 1 | |a Schwack, Wolfgang |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Rapid communications in mass spectrometry : RCM |d 1987 |g 21(2007), 12 vom: 01., Seite 1937-43 |w (DE-627)NLM012619914 |x 1097-0231 |7 nnns |
773 | 1 | 8 | |g volume:21 |g year:2007 |g number:12 |g day:01 |g pages:1937-43 |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 21 |j 2007 |e 12 |b 01 |h 1937-43 |