High-level expression and purification of recombinant human catalase in Pichia pastoris
Catalase is one of the antioxidant enzymes and is involved in many pathophysiologic processes and human diseases. This study focused on high-level expression and purification of recombinant catalase in Pichia pastoris. The cDNA encoding catalase was cloned by RT-PCR from Fetal liver of Homo sapiens. After PCR and construction of expression vector pPIC9K-CAT, human catalase was expressed highly in P. pastoris yeast SMD1168 and secreted into the culture medium. The secreted catalase was purified to a purity of 95% by ammonium sulfate fractionation, anionic exchange-chromatography, and Macro-prep Ceramic Hydroxyapatite with a overall yield of 60%. This study provides a new method for large-scale expression and purification of recombinant protein catalase.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2007 |
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| Erschienen: |
2007 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:54 |
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| Enthalten in: |
Protein expression and purification - 54(2007), 1 vom: 01. Juli, Seite 24-9 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shi, Xun-Long [VerfasserIn] |
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| Themen: |
Catalase |
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| Anmerkungen: |
Date Completed 16.07.2007 Date Revised 30.04.2007 published: Print-Electronic Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM169427439 |
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| 100 | 1 | |a Shi, Xun-Long |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a High-level expression and purification of recombinant human catalase in Pichia pastoris |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Catalase is one of the antioxidant enzymes and is involved in many pathophysiologic processes and human diseases. This study focused on high-level expression and purification of recombinant catalase in Pichia pastoris. The cDNA encoding catalase was cloned by RT-PCR from Fetal liver of Homo sapiens. After PCR and construction of expression vector pPIC9K-CAT, human catalase was expressed highly in P. pastoris yeast SMD1168 and secreted into the culture medium. The secreted catalase was purified to a purity of 95% by ammonium sulfate fractionation, anionic exchange-chromatography, and Macro-prep Ceramic Hydroxyapatite with a overall yield of 60%. This study provides a new method for large-scale expression and purification of recombinant protein catalase | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA, Complementary |2 NLM | |
| 650 | 7 | |a Recombinant Proteins |2 NLM | |
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| 700 | 1 | |a Feng, Mei-Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Zhi-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong, Jiang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Pei |e verfasserin |4 aut | |
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