Molecular cloning and functional analysis of goose interferon gamma
The cDNA for goose interferon gamma (goIFN-gamma) was cloned from PHA-stimulated goose peripheral blood mononuclear cells (PBMCs) by RT-PCR. This cDNA encodes a 19-amino acid signal peptide and a 145-amino acid mature protein, which shares a high homology with duck IFN-gamma. Recombinant mature goose interferon gamma (rgoIFN-gamma) generated from both prokaryotic and eukaryotic expression systems effectively inhibited the replication of goose paramyxovirus and recombinant vesicular stomatitis virus in vitro. These antiviral activities were abrogated by rabbit anti-rgoIFN-gamma antibodies in vitro. Furthermore, rgoIFN-gamma stimulated goose peritoneal macrophages to produce nitric oxide (NO) in vitro, demonstrating its macrophage activating factor (MAF) activity. Therefore, the availability of bioactive rgoIFN-gamma and its specific antibodies provides valuable tools for studying T cell immunity in geese.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2007 |
|---|---|
| Erschienen: |
2007 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:117 |
|---|---|
| Enthalten in: |
Veterinary immunology and immunopathology - 117(2007), 1-2 vom: 15. Mai, Seite 67-74 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Li, Hong-Tao [VerfasserIn] |
|---|
| Themen: |
82115-62-6 |
|---|
| Anmerkungen: |
Date Completed 17.07.2007 Date Revised 21.11.2008 published: Print-Electronic GENBANK: AY524421 Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM168751097 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM168751097 | ||
| 003 | DE-627 | ||
| 005 | 20231223115816.0 | ||
| 007 | tu | ||
| 008 | 231223s2007 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0563.xml |
| 035 | |a (DE-627)NLM168751097 | ||
| 035 | |a (NLM)17336393 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Li, Hong-Tao |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Molecular cloning and functional analysis of goose interferon gamma |
| 264 | 1 | |c 2007 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 17.07.2007 | ||
| 500 | |a Date Revised 21.11.2008 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a GENBANK: AY524421 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The cDNA for goose interferon gamma (goIFN-gamma) was cloned from PHA-stimulated goose peripheral blood mononuclear cells (PBMCs) by RT-PCR. This cDNA encodes a 19-amino acid signal peptide and a 145-amino acid mature protein, which shares a high homology with duck IFN-gamma. Recombinant mature goose interferon gamma (rgoIFN-gamma) generated from both prokaryotic and eukaryotic expression systems effectively inhibited the replication of goose paramyxovirus and recombinant vesicular stomatitis virus in vitro. These antiviral activities were abrogated by rabbit anti-rgoIFN-gamma antibodies in vitro. Furthermore, rgoIFN-gamma stimulated goose peritoneal macrophages to produce nitric oxide (NO) in vitro, demonstrating its macrophage activating factor (MAF) activity. Therefore, the availability of bioactive rgoIFN-gamma and its specific antibodies provides valuable tools for studying T cell immunity in geese | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Recombinant Proteins |2 NLM | |
| 650 | 7 | |a Interferon-gamma |2 NLM | |
| 650 | 7 | |a 82115-62-6 |2 NLM | |
| 700 | 1 | |a Ma, Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Mi, Jing-Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Jin, Hong-Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Li-Na |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jun-Wei |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Veterinary immunology and immunopathology |d 1979 |g 117(2007), 1-2 vom: 15. Mai, Seite 67-74 |w (DE-627)NLM012599875 |x 1873-2534 |7 nnns |
| 773 | 1 | 8 | |g volume:117 |g year:2007 |g number:1-2 |g day:15 |g month:05 |g pages:67-74 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 117 |j 2007 |e 1-2 |b 15 |c 05 |h 67-74 | ||