Determination of xanthohumol in hops (Humulus lupulus L.) by nonaqueous CE
Xanthohumol (XN) is a prenylated chalcone with antimutagenic and anticancer activity from hops. A nonaqueous reverse polarity capillary electrophoretic method for the determination of XN in hop extract was developed and validated. The optimal parameters were a 64.5 cm long fused-silica capillary with 50 microm id at 25 degrees C; 30 kV negative voltage (anode at detector side of the capillary); nonaqueous buffer with 75 mM NaOH and 50 mM boric acid in methanol; hydrodynamical injection with 10 mbar for 40 s; and detection at 440 nm. XN, isoxanthohumol (IX), colupulone, adlupulone, and n-lupulone were well resolved on the electropherogram. The LOD for XN was 0.05 mg/L and RSD for peak area was below 3%. The amount of XN in different samples of hop pellets varied from 0.14 to 0.42%.
Medienart: |
Artikel |
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Erscheinungsjahr: |
2007 |
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Erschienen: |
2007 |
Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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Enthalten in: |
Electrophoresis - 28(2007), 6 vom: 10. März, Seite 965-9 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Kac, Javor [VerfasserIn] |
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Anmerkungen: |
Date Completed 30.07.2007 Date Revised 10.12.2019 published: Print Citation Status MEDLINE |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM168496224 |
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245 | 1 | 0 | |a Determination of xanthohumol in hops (Humulus lupulus L.) by nonaqueous CE |
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520 | |a Xanthohumol (XN) is a prenylated chalcone with antimutagenic and anticancer activity from hops. A nonaqueous reverse polarity capillary electrophoretic method for the determination of XN in hop extract was developed and validated. The optimal parameters were a 64.5 cm long fused-silica capillary with 50 microm id at 25 degrees C; 30 kV negative voltage (anode at detector side of the capillary); nonaqueous buffer with 75 mM NaOH and 50 mM boric acid in methanol; hydrodynamical injection with 10 mbar for 40 s; and detection at 440 nm. XN, isoxanthohumol (IX), colupulone, adlupulone, and n-lupulone were well resolved on the electropherogram. The LOD for XN was 0.05 mg/L and RSD for peak area was below 3%. The amount of XN in different samples of hop pellets varied from 0.14 to 0.42% | ||
650 | 4 | |a Evaluation Study | |
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