Genotypic resistance of archived and circulating viral strains in the blood of treated HIV-infected individuals
The aim of this study was to evaluate patterns of antiretroviral resistance of HIV-1 in peripheral blood mononuclear cells (PBMCs) and in the plasma of patients whose therapeutic regimen is failing. Plasma and PBMC samples were collected from 95 HIV-infected patients undergoing long-term treatment. Genotyping of the reverse transcriptase (RT) and protease genes of HIV-1 was undertaken using the fluorescent dideoxy-terminator method. Comparison of the amino acid sequence of the RT and protease genes in cell-associated variants of HIV-1 with that of the plasma revealed that 62 of the 95 patients' samples tested exhibited different genotypic resistance patterns (discordant samples [DSs]). In 27% of samples, the patterns of resistance detected were concordant in both compartments. In 51% of DSs, the greatest number of mutations was found in plasma; however, in 37% of DSs, greater numbers of mutations were found in PBMC DNA. The HIV mutation patterns detected in plasma do not necessarily reflect those found in the cell-associated compartment. The observation that the cellular compartment may contain an archive of the resistance variant makes this reservoir an interesting substrate for analysis of the "resistance potential" in a given patient.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2007 |
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| Erschienen: |
2007 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
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| Enthalten in: |
Journal of acquired immune deficiency syndromes (1999) - 44(2007), 5 vom: 15. Apr., Seite 518-24 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Turriziani, Ombretta [VerfasserIn] |
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| Themen: |
Anti-HIV Agents |
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| Anmerkungen: |
Date Completed 16.05.2007 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM167711164 |
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| 100 | 1 | |a Turriziani, Ombretta |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Genotypic resistance of archived and circulating viral strains in the blood of treated HIV-infected individuals |
| 264 | 1 | |c 2007 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
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| 500 | |a Date Completed 16.05.2007 | ||
| 500 | |a Date Revised 30.09.2020 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The aim of this study was to evaluate patterns of antiretroviral resistance of HIV-1 in peripheral blood mononuclear cells (PBMCs) and in the plasma of patients whose therapeutic regimen is failing. Plasma and PBMC samples were collected from 95 HIV-infected patients undergoing long-term treatment. Genotyping of the reverse transcriptase (RT) and protease genes of HIV-1 was undertaken using the fluorescent dideoxy-terminator method. Comparison of the amino acid sequence of the RT and protease genes in cell-associated variants of HIV-1 with that of the plasma revealed that 62 of the 95 patients' samples tested exhibited different genotypic resistance patterns (discordant samples [DSs]). In 27% of samples, the patterns of resistance detected were concordant in both compartments. In 51% of DSs, the greatest number of mutations was found in plasma; however, in 37% of DSs, greater numbers of mutations were found in PBMC DNA. The HIV mutation patterns detected in plasma do not necessarily reflect those found in the cell-associated compartment. The observation that the cellular compartment may contain an archive of the resistance variant makes this reservoir an interesting substrate for analysis of the "resistance potential" in a given patient | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Anti-HIV Agents |2 NLM | |
| 650 | 7 | |a RNA, Viral |2 NLM | |
| 650 | 7 | |a HIV Reverse Transcriptase |2 NLM | |
| 650 | 7 | |a EC 2.7.7.49 |2 NLM | |
| 650 | 7 | |a HIV Protease |2 NLM | |
| 650 | 7 | |a EC 3.4.23.- |2 NLM | |
| 700 | 1 | |a Bucci, Mauro |e verfasserin |4 aut | |
| 700 | 1 | |a Stano, Armando |e verfasserin |4 aut | |
| 700 | 1 | |a Scagnolari, Carolina |e verfasserin |4 aut | |
| 700 | 1 | |a Bellomi, Francesca |e verfasserin |4 aut | |
| 700 | 1 | |a Fimiani, Caterina |e verfasserin |4 aut | |
| 700 | 1 | |a Mezzaroma, Ivano |e verfasserin |4 aut | |
| 700 | 1 | |a D'Ettorre, Gabriella |e verfasserin |4 aut | |
| 700 | 1 | |a Brogi, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Vullo, Vincenzo |e verfasserin |4 aut | |
| 700 | 1 | |a Antonelli, Guido |e verfasserin |4 aut | |
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