Site-specific labeling of DNA-protein conjugates by means of expressed protein ligation
Site-specific bioconjugation of protein thioesters with a DNA oligonucleotide was achieved by Expressed Protein Ligation (EPL) and the new thiol group formed upon EPL in the conjugate was selectively coupled with small molecule labels using maleimide chemistry.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2007 |
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| Erschienen: |
2007 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2007 |
|---|---|
| Enthalten in: |
Chemical communications (Cambridge, England) - (2007), 4 vom: 28. Jan., Seite 353-5 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Lovrinovic, Marina [VerfasserIn] |
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| Themen: |
9007-49-2 |
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| Anmerkungen: |
Date Completed 26.03.2007 Date Revised 18.11.2010 published: Print-Electronic Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM167677624 |
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| 245 | 1 | 0 | |a Site-specific labeling of DNA-protein conjugates by means of expressed protein ligation |
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| 500 | |a Date Revised 18.11.2010 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Site-specific bioconjugation of protein thioesters with a DNA oligonucleotide was achieved by Expressed Protein Ligation (EPL) and the new thiol group formed upon EPL in the conjugate was selectively coupled with small molecule labels using maleimide chemistry | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Schröder, Hendrik |e verfasserin |4 aut | |
| 700 | 1 | |a Niemeyer, Christof M |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Chemical communications (Cambridge, England) |d 1998 |g (2007), 4 vom: 28. Jan., Seite 353-5 |w (DE-627)NLM095512322 |x 1364-548X |7 nnns |
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