Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks
In spite of strong evidence that the nucleus is a highly organized organelle, a consensus on basic principles of the global nuclear architecture has not so far been achieved. The chromosome territory-interchromatin compartment (CT-IC) model postulates an IC which expands between chromatin domains both in the interior and the periphery of CT. Other models, however, dispute the existence of the IC and claim that numerous chromatin loops expand between and within CTs. The present study was undertaken to resolve these conflicting views. (1) We demonstrate that most chromatin exists in the form of higher-order chromatin domains with a compaction level at least 10 times above the level of extended 30 nm chromatin fibers. A similar compaction level was obtained in a detailed analysis of a particularly gene-dense chromosome region on HSA 11, which often expanded from its CT as a finger-like chromatin protrusion. (2) We further applied an approach which allows the experimental manipulation of both chromatin condensation and the width of IC channels in a fully reversible manner. These experiments, together with electron microscopic observations, demonstrate the existence of the IC as a dynamic, structurally distinct nuclear compartment, which is functionally linked with the chromatin compartment.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2006 |
|---|---|
| Erschienen: |
2006 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
|---|---|
| Enthalten in: |
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology - 14(2006), 7 vom: 16., Seite 707-33 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Albiez, Heiner [VerfasserIn] |
|---|
| Themen: |
63231-63-0 |
|---|
| Anmerkungen: |
Date Completed 12.02.2007 Date Revised 16.07.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM166686247 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM166686247 | ||
| 003 | DE-627 | ||
| 005 | 20231223111327.0 | ||
| 007 | tu | ||
| 008 | 231223s2006 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0556.xml |
| 035 | |a (DE-627)NLM166686247 | ||
| 035 | |a (NLM)17115328 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Albiez, Heiner |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks |
| 264 | 1 | |c 2006 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 12.02.2007 | ||
| 500 | |a Date Revised 16.07.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a In spite of strong evidence that the nucleus is a highly organized organelle, a consensus on basic principles of the global nuclear architecture has not so far been achieved. The chromosome territory-interchromatin compartment (CT-IC) model postulates an IC which expands between chromatin domains both in the interior and the periphery of CT. Other models, however, dispute the existence of the IC and claim that numerous chromatin loops expand between and within CTs. The present study was undertaken to resolve these conflicting views. (1) We demonstrate that most chromatin exists in the form of higher-order chromatin domains with a compaction level at least 10 times above the level of extended 30 nm chromatin fibers. A similar compaction level was obtained in a detailed analysis of a particularly gene-dense chromosome region on HSA 11, which often expanded from its CT as a finger-like chromatin protrusion. (2) We further applied an approach which allows the experimental manipulation of both chromatin condensation and the width of IC channels in a fully reversible manner. These experiments, together with electron microscopic observations, demonstrate the existence of the IC as a dynamic, structurally distinct nuclear compartment, which is functionally linked with the chromatin compartment | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Chromatin |2 NLM | |
| 650 | 7 | |a RNA |2 NLM | |
| 650 | 7 | |a 63231-63-0 |2 NLM | |
| 650 | 7 | |a DNA |2 NLM | |
| 650 | 7 | |a 9007-49-2 |2 NLM | |
| 650 | 7 | |a RNA Polymerase II |2 NLM | |
| 650 | 7 | |a EC 2.7.7.- |2 NLM | |
| 700 | 1 | |a Cremer, Marion |e verfasserin |4 aut | |
| 700 | 1 | |a Tiberi, Cinzia |e verfasserin |4 aut | |
| 700 | 1 | |a Vecchio, Lorella |e verfasserin |4 aut | |
| 700 | 1 | |a Schermelleh, Lothar |e verfasserin |4 aut | |
| 700 | 1 | |a Dittrich, Sandra |e verfasserin |4 aut | |
| 700 | 1 | |a Küpper, Katrin |e verfasserin |4 aut | |
| 700 | 1 | |a Joffe, Boris |e verfasserin |4 aut | |
| 700 | 1 | |a Thormeyer, Tobias |e verfasserin |4 aut | |
| 700 | 1 | |a von Hase, Johann |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Siwei |e verfasserin |4 aut | |
| 700 | 1 | |a Rohr, Karl |e verfasserin |4 aut | |
| 700 | 1 | |a Leonhardt, Heinrich |e verfasserin |4 aut | |
| 700 | 1 | |a Solovei, Irina |e verfasserin |4 aut | |
| 700 | 1 | |a Cremer, Christoph |e verfasserin |4 aut | |
| 700 | 1 | |a Fakan, Stanislav |e verfasserin |4 aut | |
| 700 | 1 | |a Cremer, Thomas |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology |d 1993 |g 14(2006), 7 vom: 16., Seite 707-33 |w (DE-627)NLM074876163 |x 1573-6849 |7 nnns |
| 773 | 1 | 8 | |g volume:14 |g year:2006 |g number:7 |g day:16 |g pages:707-33 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 14 |j 2006 |e 7 |b 16 |h 707-33 | ||