Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis
Because of the outstanding separating capabilities of two-dimensional electrophoresis for complete proteins, it would be advantageous to be able to apply it to all types of proteins. Unfortunately, severe solubility problems hamper the analysis of many classes of proteins, but especially membrane proteins. These problems arise mainly in the extraction and isoelectric focusing steps, and solutions are sought to improve protein solubility under the conditions prevailing during isoelectric focusing. These solutions deal mainly with chaotropes and new detergents, which are both able to enhance protein solubility. The input of these compounds in proteomics analysis of membrane proteins is discussed, as well as future directions.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2007 |
|---|---|
| Erschienen: |
2007 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:355 |
|---|---|
| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 355(2007) vom: 10., Seite 111-9 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Rabilloud, Thierry [VerfasserIn] |
|---|
| Themen: |
8W8T17847W |
|---|
| Anmerkungen: |
Date Completed 01.02.2007 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM166478407 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM166478407 | ||
| 003 | DE-627 | ||
| 005 | 20231223110903.0 | ||
| 007 | tu | ||
| 008 | 231223s2007 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0555.xml |
| 035 | |a (DE-627)NLM166478407 | ||
| 035 | |a (NLM)17093307 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Rabilloud, Thierry |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis |
| 264 | 1 | |c 2007 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 01.02.2007 | ||
| 500 | |a Date Revised 21.11.2013 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Because of the outstanding separating capabilities of two-dimensional electrophoresis for complete proteins, it would be advantageous to be able to apply it to all types of proteins. Unfortunately, severe solubility problems hamper the analysis of many classes of proteins, but especially membrane proteins. These problems arise mainly in the extraction and isoelectric focusing steps, and solutions are sought to improve protein solubility under the conditions prevailing during isoelectric focusing. These solutions deal mainly with chaotropes and new detergents, which are both able to enhance protein solubility. The input of these compounds in proteomics analysis of membrane proteins is discussed, as well as future directions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Detergents |2 NLM | |
| 650 | 7 | |a Membrane Proteins |2 NLM | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 650 | 7 | |a Solutions |2 NLM | |
| 650 | 7 | |a Urea |2 NLM | |
| 650 | 7 | |a 8W8T17847W |2 NLM | |
| 650 | 7 | |a Thiourea |2 NLM | |
| 650 | 7 | |a GYV9AM2QAG |2 NLM | |
| 700 | 1 | |a Luche, Sylvie |e verfasserin |4 aut | |
| 700 | 1 | |a Santoni, Véronique |e verfasserin |4 aut | |
| 700 | 1 | |a Chevallet, Mireille |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Methods in molecular biology (Clifton, N.J.) |d 1984 |g 355(2007) vom: 10., Seite 111-9 |w (DE-627)NLM074849794 |x 1940-6029 |7 nnns |
| 773 | 1 | 8 | |g volume:355 |g year:2007 |g day:10 |g pages:111-9 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 355 |j 2007 |b 10 |h 111-9 | ||