Long-term room temperature storage of high-quality embryonic stem cell genomic DNA extracted with a simple and rapid procedure
A very simple procedure for the isolation of high-quality, high-molecular-weight genomic DNA from embryonic stem cells is described. The DNA is very stable once dried and can be stored for long periods of time without refrigeration. Living cells are lysed in a sodium dodecyl sulfate and EDTA buffer containing proteinase K and then air-dried. Samples can be processed in bulk, and an individual can easily process thousands of samples for extraction and shipment on a daily basis using only common laboratory materials such as plastic ware and a multichannel pipetteman.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2006 |
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| Erschienen: |
2006 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
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| Enthalten in: |
Journal of biomolecular techniques : JBT - 17(2006), 4 vom: 01. Sept., Seite 249-51 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Tribioli, Carla [VerfasserIn] |
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| Themen: |
147336-22-9 |
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| Anmerkungen: |
Date Completed 30.11.2006 Date Revised 13.11.2018 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM165864591 |
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| 100 | 1 | |a Tribioli, Carla |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Long-term room temperature storage of high-quality embryonic stem cell genomic DNA extracted with a simple and rapid procedure |
| 264 | 1 | |c 2006 | |
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| 500 | |a Date Completed 30.11.2006 | ||
| 500 | |a Date Revised 13.11.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A very simple procedure for the isolation of high-quality, high-molecular-weight genomic DNA from embryonic stem cells is described. The DNA is very stable once dried and can be stored for long periods of time without refrigeration. Living cells are lysed in a sodium dodecyl sulfate and EDTA buffer containing proteinase K and then air-dried. Samples can be processed in bulk, and an individual can easily process thousands of samples for extraction and shipment on a daily basis using only common laboratory materials such as plastic ware and a multichannel pipetteman | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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