The Salmonella effector protein PipB2 is a linker for kinesin-1
Understanding the mechanisms of Salmonella virulence is an important challenge. The capacity of this intracellular bacterial pathogen to cause diseases depends on the expression of virulence factors including the second type III secretion system (TTSS-2), which is used to translocate into the eukaryotic cytosol a set of effector proteins that divert the biology of the host cell and shape the bacterial replicative niche. Yet little is known about the eukaryotic functions affected by individual Salmonella effectors. Here we report that the TTSS-2 effector PipB2 interacts with the kinesin light chain, a subunit of the kinesin-1 motor complex that drives anterograde transport along microtubules. Translocation of PipB2 is both necessary and sufficient for the recruitment of kinesin-1 to the membrane of the Salmonella-containing vacuole. In vivo, PipB2 contributes to the attenuation of Salmonella mutant strains in mice. Taken together, our data indicate that the TTSS-2-mediated fine-tuning of kinesin-1 activity associated with the bacterial vacuole is crucial for the virulence of Salmonella.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2006 |
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| Erschienen: |
2006 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:103 |
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| Enthalten in: |
Proceedings of the National Academy of Sciences of the United States of America - 103(2006), 36 vom: 05. Sept., Seite 13497-502 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Henry, Thomas [VerfasserIn] |
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| Themen: |
Bacterial Proteins |
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| Anmerkungen: |
Date Completed 19.10.2006 Date Revised 03.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM165059060 |
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| 100 | 1 | |a Henry, Thomas |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The Salmonella effector protein PipB2 is a linker for kinesin-1 |
| 264 | 1 | |c 2006 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 19.10.2006 | ||
| 500 | |a Date Revised 03.12.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Understanding the mechanisms of Salmonella virulence is an important challenge. The capacity of this intracellular bacterial pathogen to cause diseases depends on the expression of virulence factors including the second type III secretion system (TTSS-2), which is used to translocate into the eukaryotic cytosol a set of effector proteins that divert the biology of the host cell and shape the bacterial replicative niche. Yet little is known about the eukaryotic functions affected by individual Salmonella effectors. Here we report that the TTSS-2 effector PipB2 interacts with the kinesin light chain, a subunit of the kinesin-1 motor complex that drives anterograde transport along microtubules. Translocation of PipB2 is both necessary and sufficient for the recruitment of kinesin-1 to the membrane of the Salmonella-containing vacuole. In vivo, PipB2 contributes to the attenuation of Salmonella mutant strains in mice. Taken together, our data indicate that the TTSS-2-mediated fine-tuning of kinesin-1 activity associated with the bacterial vacuole is crucial for the virulence of Salmonella | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Intramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a PipB2 protein, Salmonella enterica |2 NLM | |
| 650 | 7 | |a Kinesins |2 NLM | |
| 650 | 7 | |a EC 3.6.4.4 |2 NLM | |
| 700 | 1 | |a Couillault, Carole |e verfasserin |4 aut | |
| 700 | 1 | |a Rockenfeller, Patrick |e verfasserin |4 aut | |
| 700 | 1 | |a Boucrot, Emmanuel |e verfasserin |4 aut | |
| 700 | 1 | |a Dumont, Audrey |e verfasserin |4 aut | |
| 700 | 1 | |a Schroeder, Nina |e verfasserin |4 aut | |
| 700 | 1 | |a Hermant, Aurélie |e verfasserin |4 aut | |
| 700 | 1 | |a Knodler, Leigh A |e verfasserin |4 aut | |
| 700 | 1 | |a Lecine, Patrick |e verfasserin |4 aut | |
| 700 | 1 | |a Steele-Mortimer, Olivia |e verfasserin |4 aut | |
| 700 | 1 | |a Borg, Jean-Paul |e verfasserin |4 aut | |
| 700 | 1 | |a Gorvel, Jean-Pierre |e verfasserin |4 aut | |
| 700 | 1 | |a Méresse, Stéphane |e verfasserin |4 aut | |
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