Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography
A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4:1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their (1)H NMR and (13)C NMR spectra.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2006 |
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| Erschienen: |
2006 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1127 |
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| Enthalten in: |
Journal of chromatography. A - 1127(2006), 1-2 vom: 15. Sept., Seite 298-301 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Han, Quan Bin [VerfasserIn] |
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| Themen: |
8N585K83U2 |
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| Anmerkungen: |
Date Completed 12.10.2006 Date Revised 24.11.2016 published: Print-Electronic Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM164570799 |
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| 100 | 1 | |a Han, Quan Bin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography |
| 264 | 1 | |c 2006 | |
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| 500 | |a Date Completed 12.10.2006 | ||
| 500 | |a Date Revised 24.11.2016 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4:1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their (1)H NMR and (13)C NMR spectra | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Xanthones |2 NLM | |
| 650 | 7 | |a gambogic acid |2 NLM | |
| 650 | 7 | |a 8N585K83U2 |2 NLM | |
| 700 | 1 | |a Song, Jing Zheng |e verfasserin |4 aut | |
| 700 | 1 | |a Qiao, Chun Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Lina |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Hong Xi |e verfasserin |4 aut | |
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