Keratan sulfate and chondroitin/dermatan sulfate in maximally recovered hypocellular stromal interface scars of postmortem human LASIK corneas
PURPOSE: To analyze the amounts and distributions of nonsulfated and sulfated keratan sulfate (KS) and chondroitin/dermatan sulfate (CS/DS) disaccharides in the interface wound of human postmortem LASIK corneas in comparison with normal control corneas.
METHODS: Corneal stromal tissue samples from central and paracentral hypocellular primitive stromal interface scars of human LASIK corneas and from similar regions of normal control corneas were collected by laser capture microdissection (LCM) and subsequently were digested with specific glycosidase enzymes. Digests were directly analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS).
RESULTS: Concentrations of both monosulfated GlcNAc(6S)-beta-1,3-Gal (MSD2) and disulfated Gal (6S)-beta-1,4-GlcNAc(6S) (DSD) KS disaccharides from the LASIK interface scars were significantly lower than in normal control corneal stromas. No significant difference was found for the concentration of nonsulfated (NSD) KS disaccharides in LASIK interface scars compared with normal controls. The concentration of DeltaUA-beta-1,3-GalNAc(6S) (Deltadi-6S) CS/DS disaccharides from the LASIK interface scar was significantly higher than normal corneal stroma, whereas concentrations of DeltaUA-beta-1,3-GalNAc(4S) (Deltadi-4S) and nonsulfated Deltadi-0S CS/DS disaccharides demonstrated no significant differences from normal corneas.
CONCLUSIONS: The profiles of KS and CS/DS disaccharides in LASIK interface scars are significantly different from those in normal cornea stromal tissue, as revealed by LCM and ESI-MS/MS.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2006 |
|---|---|
| Erschienen: |
2006 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:47 |
|---|---|
| Enthalten in: |
Investigative ophthalmology & visual science - 47(2006), 6 vom: 24. Juni, Seite 2390-6 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zhang, Yuntao [VerfasserIn] |
|---|
| Themen: |
24967-94-0 |
|---|
| Anmerkungen: |
Date Completed 03.07.2006 Date Revised 14.11.2007 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM163025371 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM163025371 | ||
| 003 | DE-627 | ||
| 005 | 20231223095457.0 | ||
| 007 | tu | ||
| 008 | 231223s2006 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0544.xml |
| 035 | |a (DE-627)NLM163025371 | ||
| 035 | |a (NLM)16723448 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zhang, Yuntao |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Keratan sulfate and chondroitin/dermatan sulfate in maximally recovered hypocellular stromal interface scars of postmortem human LASIK corneas |
| 264 | 1 | |c 2006 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 03.07.2006 | ||
| 500 | |a Date Revised 14.11.2007 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a PURPOSE: To analyze the amounts and distributions of nonsulfated and sulfated keratan sulfate (KS) and chondroitin/dermatan sulfate (CS/DS) disaccharides in the interface wound of human postmortem LASIK corneas in comparison with normal control corneas | ||
| 520 | |a METHODS: Corneal stromal tissue samples from central and paracentral hypocellular primitive stromal interface scars of human LASIK corneas and from similar regions of normal control corneas were collected by laser capture microdissection (LCM) and subsequently were digested with specific glycosidase enzymes. Digests were directly analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS) | ||
| 520 | |a RESULTS: Concentrations of both monosulfated GlcNAc(6S)-beta-1,3-Gal (MSD2) and disulfated Gal (6S)-beta-1,4-GlcNAc(6S) (DSD) KS disaccharides from the LASIK interface scars were significantly lower than in normal control corneal stromas. No significant difference was found for the concentration of nonsulfated (NSD) KS disaccharides in LASIK interface scars compared with normal controls. The concentration of DeltaUA-beta-1,3-GalNAc(6S) (Deltadi-6S) CS/DS disaccharides from the LASIK interface scar was significantly higher than normal corneal stroma, whereas concentrations of DeltaUA-beta-1,3-GalNAc(4S) (Deltadi-4S) and nonsulfated Deltadi-0S CS/DS disaccharides demonstrated no significant differences from normal corneas | ||
| 520 | |a CONCLUSIONS: The profiles of KS and CS/DS disaccharides in LASIK interface scars are significantly different from those in normal cornea stromal tissue, as revealed by LCM and ESI-MS/MS | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Dermatan Sulfate |2 NLM | |
| 650 | 7 | |a 24967-94-0 |2 NLM | |
| 650 | 7 | |a Chondroitin Sulfates |2 NLM | |
| 650 | 7 | |a 9007-28-7 |2 NLM | |
| 650 | 7 | |a Keratan Sulfate |2 NLM | |
| 650 | 7 | |a 9056-36-4 |2 NLM | |
| 700 | 1 | |a Schmack, Ingo |e verfasserin |4 aut | |
| 700 | 1 | |a Dawson, Daniel G |e verfasserin |4 aut | |
| 700 | 1 | |a Grossniklaus, Hans E |e verfasserin |4 aut | |
| 700 | 1 | |a Conrad, Abigail H |e verfasserin |4 aut | |
| 700 | 1 | |a Kariya, Yutaka |e verfasserin |4 aut | |
| 700 | 1 | |a Suzuki, Kiyoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Edelhauser, Henry F |e verfasserin |4 aut | |
| 700 | 1 | |a Conrad, Gary W |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Investigative ophthalmology & visual science |d 1985 |g 47(2006), 6 vom: 24. Juni, Seite 2390-6 |w (DE-627)NLM000138320 |x 1552-5783 |7 nnns |
| 773 | 1 | 8 | |g volume:47 |g year:2006 |g number:6 |g day:24 |g month:06 |g pages:2390-6 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 47 |j 2006 |e 6 |b 24 |c 06 |h 2390-6 | ||