Molecular cloning and characterisation of two homologues of Mannose-Binding Lectin in rainbow trout
Mannose-binding lectin (MBL) is a C-type lectin which participates in the innate immune system as an activator of the complement system and as opsonin after binding to certain carbohydrate structures on microorganisms and pathogens. C-type lectins are all Ca(2+)-dependent molecules and they share a tightly folded carbohydrate recognition domain (CRD). In this report the isolation and characterisation of cDNA transcripts encoding two mannose-binding lectin isoforms MBL-1 and MBL-2 from rainbow trout (Oncorhynchus mykiss) is presented. The deduced amino acid sequences of trout MBL-1 and MBL-2 (185 and 186 aa, respectively) present 83% identity to each other, exhibiting the highest identity score 46, 46 and 42% with the Atlantic salmon, shishamo smelt and zebrafish counterparts, respectively. The identity to birds and mammalian MBLs ranges from 25 to 33%. The trout MBL-1 and MBL-2 contain the EPN motif of mannose-binding C-type lectins, important for mannose specificity and they are expressed exclusively in liver and spleen, respectively.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2006 |
|---|---|
| Erschienen: |
2006 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
|---|---|
| Enthalten in: |
Fish & shellfish immunology - 21(2006), 3 vom: 21. Sept., Seite 305-14 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Nikolakopoulou, Konstantina [VerfasserIn] |
|---|
| Themen: |
Actins |
|---|
| Anmerkungen: |
Date Completed 18.08.2006 Date Revised 15.11.2006 published: Print-Electronic GENBANK: AF363271, AM041212 Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM161328199 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM161328199 | ||
| 003 | DE-627 | ||
| 005 | 20231223091946.0 | ||
| 007 | tu | ||
| 008 | 231223s2006 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0538.xml |
| 035 | |a (DE-627)NLM161328199 | ||
| 035 | |a (NLM)16542855 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Nikolakopoulou, Konstantina |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Molecular cloning and characterisation of two homologues of Mannose-Binding Lectin in rainbow trout |
| 264 | 1 | |c 2006 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 18.08.2006 | ||
| 500 | |a Date Revised 15.11.2006 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a GENBANK: AF363271, AM041212 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Mannose-binding lectin (MBL) is a C-type lectin which participates in the innate immune system as an activator of the complement system and as opsonin after binding to certain carbohydrate structures on microorganisms and pathogens. C-type lectins are all Ca(2+)-dependent molecules and they share a tightly folded carbohydrate recognition domain (CRD). In this report the isolation and characterisation of cDNA transcripts encoding two mannose-binding lectin isoforms MBL-1 and MBL-2 from rainbow trout (Oncorhynchus mykiss) is presented. The deduced amino acid sequences of trout MBL-1 and MBL-2 (185 and 186 aa, respectively) present 83% identity to each other, exhibiting the highest identity score 46, 46 and 42% with the Atlantic salmon, shishamo smelt and zebrafish counterparts, respectively. The identity to birds and mammalian MBLs ranges from 25 to 33%. The trout MBL-1 and MBL-2 contain the EPN motif of mannose-binding C-type lectins, important for mannose specificity and they are expressed exclusively in liver and spleen, respectively | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Actins |2 NLM | |
| 650 | 7 | |a DNA Primers |2 NLM | |
| 650 | 7 | |a DNA, Complementary |2 NLM | |
| 650 | 7 | |a Mannose-Binding Lectins |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 700 | 1 | |a Zarkadis, Ioannis K |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Fish & shellfish immunology |d 1997 |g 21(2006), 3 vom: 21. Sept., Seite 305-14 |w (DE-627)NLM094469725 |x 1095-9947 |7 nnns |
| 773 | 1 | 8 | |g volume:21 |g year:2006 |g number:3 |g day:21 |g month:09 |g pages:305-14 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 21 |j 2006 |e 3 |b 21 |c 09 |h 305-14 | ||