Immunization with dendritic cells retrovirally transduced with mycobacterial antigen 85A gene elicits the specific cellular immunity including cytotoxic T-lymphocyte activity specific to an epitope on antigen 85A
In the present study, we evaluated antigen 85A (Ag85A) gene-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A gene and injected to BALB/c mice intravenously. The DC vaccine was capable of inducing purified protein derivative (PPD)- and the antigen-specific spleen cell proliferation and IFN-gamma production from both CD4+ and CD8+ T cells in spleens of the immune mice. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) and IFN-gamma-producing cells specific for a 9-mer CTL epitope on Ag85A molecule. This eliciting cellular immunity led to protection against wasting disease due to M. tuberculosis infection and induction of moderate bacterial clearance.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2006 |
|---|---|
| Erschienen: |
2006 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
|---|---|
| Enthalten in: |
Vaccine - 24(2006), 12 vom: 15. März, Seite 2110-9 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Nakano, Hideki [VerfasserIn] |
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| Themen: |
Antigen 85, Mycobacterium bovis |
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| Anmerkungen: |
Date Completed 28.06.2006 Date Revised 15.11.2006 published: Print-Electronic Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM159542014 |
|---|
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| 100 | 1 | |a Nakano, Hideki |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Immunization with dendritic cells retrovirally transduced with mycobacterial antigen 85A gene elicits the specific cellular immunity including cytotoxic T-lymphocyte activity specific to an epitope on antigen 85A |
| 264 | 1 | |c 2006 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 28.06.2006 | ||
| 500 | |a Date Revised 15.11.2006 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a In the present study, we evaluated antigen 85A (Ag85A) gene-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A gene and injected to BALB/c mice intravenously. The DC vaccine was capable of inducing purified protein derivative (PPD)- and the antigen-specific spleen cell proliferation and IFN-gamma production from both CD4+ and CD8+ T cells in spleens of the immune mice. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) and IFN-gamma-producing cells specific for a 9-mer CTL epitope on Ag85A molecule. This eliciting cellular immunity led to protection against wasting disease due to M. tuberculosis infection and induction of moderate bacterial clearance | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Antigens, Bacterial |2 NLM | |
| 650 | 7 | |a Bacterial Vaccines |2 NLM | |
| 650 | 7 | |a Epitopes, T-Lymphocyte |2 NLM | |
| 650 | 7 | |a antigen 85, Mycobacterium bovis |2 NLM | |
| 700 | 1 | |a Nagata, Toshi |e verfasserin |4 aut | |
| 700 | 1 | |a Suda, Takafumi |e verfasserin |4 aut | |
| 700 | 1 | |a Tanaka, Takao |e verfasserin |4 aut | |
| 700 | 1 | |a Aoshi, Taiki |e verfasserin |4 aut | |
| 700 | 1 | |a Uchijima, Masato |e verfasserin |4 aut | |
| 700 | 1 | |a Kuwayama, Sachiko |e verfasserin |4 aut | |
| 700 | 1 | |a Kanamaru, Noriko |e verfasserin |4 aut | |
| 700 | 1 | |a Chida, Kingo |e verfasserin |4 aut | |
| 700 | 1 | |a Nakamura, Hirotoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Okada, Masaji |e verfasserin |4 aut | |
| 700 | 1 | |a Koide, Yukio |e verfasserin |4 aut | |
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