Prevalence of pfcrt point mutations and level of chloroquine resistance in Plasmodium falciparum isolates from Africa
The development in Plasmodium falciparum of the resistance to chloroquine (CQ) constitutes a public health priority, due to its direct influence in childhood mortality. The molecular basis for CQ resistance (CQR) is still unclear but, recently, a new relevant gene, named pfcrt, with several point mutations was identified in P. falciparum. Two mutations, K76T and A220S, have been considered crucial for CQR in further studies, making the pfcrt a good candidate as determinant for CQR in P. falciparum. To contribute to this topic, we have undertaken a molecular screening on 164 P. falciparum isolates from Africa: 120 isolates were Italian imported malaria cases, 27 and 17 isolates were from a school-children survey from Congo and Tanzania, respectively. In vitro tests (pLDH and WHO-Mark III tests) for CQ sensitivity have been also carried out on 28 plasmodial isolates and results compared to those obtained by molecular analysis in the same isolates. The SVIET pfcrt haplotype has been identified in the samples from Congo, and this is the first time that this haplotype is detected in Africa. Our results give further evidence to the reliability of the 76T (and the linked 74I-75E) pfcrt point mutation as molecular marker for CQR.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2006 |
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| Erschienen: |
2006 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
|---|---|
| Enthalten in: |
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 6(2006), 4 vom: 01. Juli, Seite 262-8 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Severini, Carlo [VerfasserIn] |
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| Themen: |
886U3H6UFF |
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| Anmerkungen: |
Date Completed 30.08.2006 Date Revised 21.11.2013 published: Print-Electronic Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM157694062 |
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| 100 | 1 | |a Severini, Carlo |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Prevalence of pfcrt point mutations and level of chloroquine resistance in Plasmodium falciparum isolates from Africa |
| 264 | 1 | |c 2006 | |
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| 500 | |a Date Revised 21.11.2013 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The development in Plasmodium falciparum of the resistance to chloroquine (CQ) constitutes a public health priority, due to its direct influence in childhood mortality. The molecular basis for CQ resistance (CQR) is still unclear but, recently, a new relevant gene, named pfcrt, with several point mutations was identified in P. falciparum. Two mutations, K76T and A220S, have been considered crucial for CQR in further studies, making the pfcrt a good candidate as determinant for CQR in P. falciparum. To contribute to this topic, we have undertaken a molecular screening on 164 P. falciparum isolates from Africa: 120 isolates were Italian imported malaria cases, 27 and 17 isolates were from a school-children survey from Congo and Tanzania, respectively. In vitro tests (pLDH and WHO-Mark III tests) for CQ sensitivity have been also carried out on 28 plasmodial isolates and results compared to those obtained by molecular analysis in the same isolates. The SVIET pfcrt haplotype has been identified in the samples from Congo, and this is the first time that this haplotype is detected in Africa. Our results give further evidence to the reliability of the 76T (and the linked 74I-75E) pfcrt point mutation as molecular marker for CQR | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Antimalarials |2 NLM | |
| 650 | 7 | |a Membrane Proteins |2 NLM | |
| 650 | 7 | |a Membrane Transport Proteins |2 NLM | |
| 650 | 7 | |a PfCRT protein, Plasmodium falciparum |2 NLM | |
| 650 | 7 | |a Protozoan Proteins |2 NLM | |
| 650 | 7 | |a Chloroquine |2 NLM | |
| 650 | 7 | |a 886U3H6UFF |2 NLM | |
| 700 | 1 | |a Menegon, Michela |e verfasserin |4 aut | |
| 700 | 1 | |a Sannella, Anna Rosa |e verfasserin |4 aut | |
| 700 | 1 | |a Paglia, Maria Grazia |e verfasserin |4 aut | |
| 700 | 1 | |a Narciso, Pasquale |e verfasserin |4 aut | |
| 700 | 1 | |a Matteelli, Alberto |e verfasserin |4 aut | |
| 700 | 1 | |a Gulletta, Maurizio |e verfasserin |4 aut | |
| 700 | 1 | |a Caramello, Pietro |e verfasserin |4 aut | |
| 700 | 1 | |a Canta, Francesca |e verfasserin |4 aut | |
| 700 | 1 | |a Xayavong, Maniphet V |e verfasserin |4 aut | |
| 700 | 1 | |a Moura, Iaci N S |e verfasserin |4 aut | |
| 700 | 1 | |a Pieniazek, Norman J |e verfasserin |4 aut | |
| 700 | 1 | |a Taramelli, Donatella |e verfasserin |4 aut | |
| 700 | 1 | |a Majori, Giancarlo |e verfasserin |4 aut | |
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