A potential role for beta- and gamma-crystallins in the vascular remodeling of the eye
Copyright 2005 Wiley-Liss, Inc..
We demonstrate that expression of beta- and gamma-crystallins is associated with intraocular vessels during normal vascular development of the eye and also in the Nuc1 rat, a mutant in which the hyaloid vascular system fails to regress normally. Real-Time RT PCR, Western blot and metabolic labeling studies indicate an increased expression of beta- and gamma-crystallins in Nuc1 retina. The increased expression of crystallins was localized to the astrocytes surrounding the intraocular vessels. A similar pattern of crystallin expression was also observed in the retinal vessels during normal development. Cultured human astrocytes exposed to 3-nitropropionic acid, an established model of neuronal hypoxia, increased VEGF expression, as expected, but also increased expression of crystallins. Our data suggest that crystallins may function together with VEGF during vascular remodeling. Interestingly, in human PFV (persistent fetal vasculature) disease, where the hyaloid vasculature abnormally persists after birth, we show that astrocytes express both VEGF and crystallins.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2005 |
|---|---|
| Erschienen: |
2005 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:234 |
|---|---|
| Enthalten in: |
Developmental dynamics : an official publication of the American Association of Anatomists - 234(2005), 1 vom: 15. Sept., Seite 36-47 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zhang, Cheng [VerfasserIn] |
|---|
| Themen: |
Beta-Crystallins |
|---|
| Anmerkungen: |
Date Completed 08.05.2007 Date Revised 03.12.2007 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM156413647 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM156413647 | ||
| 003 | DE-627 | ||
| 005 | 20231223074119.0 | ||
| 007 | tu | ||
| 008 | 231223s2005 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a pubmed24n0522.xml |
| 035 | |a (DE-627)NLM156413647 | ||
| 035 | |a (NLM)16003775 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zhang, Cheng |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A potential role for beta- and gamma-crystallins in the vascular remodeling of the eye |
| 264 | 1 | |c 2005 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 08.05.2007 | ||
| 500 | |a Date Revised 03.12.2007 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright 2005 Wiley-Liss, Inc. | ||
| 520 | |a We demonstrate that expression of beta- and gamma-crystallins is associated with intraocular vessels during normal vascular development of the eye and also in the Nuc1 rat, a mutant in which the hyaloid vascular system fails to regress normally. Real-Time RT PCR, Western blot and metabolic labeling studies indicate an increased expression of beta- and gamma-crystallins in Nuc1 retina. The increased expression of crystallins was localized to the astrocytes surrounding the intraocular vessels. A similar pattern of crystallin expression was also observed in the retinal vessels during normal development. Cultured human astrocytes exposed to 3-nitropropionic acid, an established model of neuronal hypoxia, increased VEGF expression, as expected, but also increased expression of crystallins. Our data suggest that crystallins may function together with VEGF during vascular remodeling. Interestingly, in human PFV (persistent fetal vasculature) disease, where the hyaloid vasculature abnormally persists after birth, we show that astrocytes express both VEGF and crystallins | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
| 650 | 7 | |a Vascular Endothelial Growth Factor A |2 NLM | |
| 650 | 7 | |a beta-Crystallins |2 NLM | |
| 650 | 7 | |a gamma-Crystallins |2 NLM | |
| 700 | 1 | |a Gehlbach, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Gongora, Celine |e verfasserin |4 aut | |
| 700 | 1 | |a Cano, Marisol |e verfasserin |4 aut | |
| 700 | 1 | |a Fariss, Robert |e verfasserin |4 aut | |
| 700 | 1 | |a Hose, Stacey |e verfasserin |4 aut | |
| 700 | 1 | |a Nath, Avindra |e verfasserin |4 aut | |
| 700 | 1 | |a Green, William R |e verfasserin |4 aut | |
| 700 | 1 | |a Goldberg, Morton F |e verfasserin |4 aut | |
| 700 | 1 | |a Zigler, J Samuel |c Jr |e verfasserin |4 aut | |
| 700 | 1 | |a Sinha, Debasish |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Developmental dynamics : an official publication of the American Association of Anatomists |d 1994 |g 234(2005), 1 vom: 15. Sept., Seite 36-47 |w (DE-627)NLM012619930 |x 1097-0177 |7 nnns |
| 773 | 1 | 8 | |g volume:234 |g year:2005 |g number:1 |g day:15 |g month:09 |g pages:36-47 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 234 |j 2005 |e 1 |b 15 |c 09 |h 36-47 | ||