Effects of Se-depletion on glutathione peroxidase and selenoprotein W gene expression in the colon
Selenium (Se)-containing proteins have important roles in protecting cells from oxidative damage. This work investigated the effects of Se-depletion on the expression of the genes encoding selenoproteins in colonic mucosa from rats fed diets of different Se content and in human intestinal Caco-2 cells grown in Se-adequate or Se-depleted culture medium. Se-depletion produced statistically significant (P<0.05) falls in glutathione peroxidase (GPX) 1 mRNA (60-83%) and selenoprotein W mRNA (73%) levels, a small but significant fall in GPX4 mRNA (17-25%) but no significant change in GPX2. The data show that SelW expression in the colon is highly sensitive to Se-depletion.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2005 |
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| Erschienen: |
2005 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:579 |
|---|---|
| Enthalten in: |
FEBS letters - 579(2005), 3 vom: 31. Jan., Seite 792-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Pagmantidis, Vasileios [VerfasserIn] |
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| Themen: |
DNA, Complementary |
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| Anmerkungen: |
Date Completed 13.04.2005 Date Revised 30.10.2019 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM15330362X |
|---|
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| 245 | 1 | 0 | |a Effects of Se-depletion on glutathione peroxidase and selenoprotein W gene expression in the colon |
| 264 | 1 | |c 2005 | |
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| 500 | |a Date Completed 13.04.2005 | ||
| 500 | |a Date Revised 30.10.2019 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Selenium (Se)-containing proteins have important roles in protecting cells from oxidative damage. This work investigated the effects of Se-depletion on the expression of the genes encoding selenoproteins in colonic mucosa from rats fed diets of different Se content and in human intestinal Caco-2 cells grown in Se-adequate or Se-depleted culture medium. Se-depletion produced statistically significant (P<0.05) falls in glutathione peroxidase (GPX) 1 mRNA (60-83%) and selenoprotein W mRNA (73%) levels, a small but significant fall in GPX4 mRNA (17-25%) but no significant change in GPX2. The data show that SelW expression in the colon is highly sensitive to Se-depletion | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA, Complementary |2 NLM | |
| 650 | 7 | |a Proteins |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a SELENOW protein, human |2 NLM | |
| 650 | 7 | |a Selenoprotein W |2 NLM | |
| 650 | 7 | |a Selenoproteins |2 NLM | |
| 650 | 7 | |a Glutathione Peroxidase |2 NLM | |
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| 700 | 1 | |a Bermano, Giovanna |e verfasserin |4 aut | |
| 700 | 1 | |a Villette, Stephane |e verfasserin |4 aut | |
| 700 | 1 | |a Broom, Iain |e verfasserin |4 aut | |
| 700 | 1 | |a Arthur, John |e verfasserin |4 aut | |
| 700 | 1 | |a Hesketh, John |e verfasserin |4 aut | |
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