The role of matrix metalloproteinases in vascular function : implications for normal pregnancy and pre-eclampsia
OBJECTIVES: (1) To investigate the effects of plasma from non-pregnant, normal pregnant and pre-eclamptic women on myogenic tone and endothelium-dependent relaxation; (2) To investigate the role of matrix metalloproteinases (MMPs) in these parameters of vascular function.
DESIGN: Analysis of the effects of plasma from non-pregnant, normal pregnant and pre-eclamptic women on myogenic tone and endothelium-dependent relaxation, and the effects of MMP inhibition on these parameters.
SETTING: University of Alberta, Edmonton, Alberta, Canada.
SAMPLES: Pooled plasma from non-pregnant (n= 9), normal pregnant (n= 6) and pre-eclamptic women (n= 12).
METHODS: Mesenteric arteries from virgin female C57BL/6J mice were exposed to pooled plasma from non-pregnant, normal pregnant and pre-eclamptic women in the absence and presence of GM6001 (5 microM; MMP inhibitor), and arterial function was assessed.
MAIN OUTCOME MEASURES: Myogenic tone and endothelium-dependent relaxation.
RESULTS: Plasma of women with pre-eclampsia, and interestingly, plasma of non-pregnant women, significantly enhanced myogenic tone at pressures > or =70 mmHg (P < 0.001) and blunted the maximum relaxation to methacholine (P < 0.001; P < 0.005) in isolated arteries. GM6001 altered response only in vessels exposed to the plasma of women with pre-eclampsia; however, contrary to our hypothesis, this further enhanced myogenic tone and further abrogated relaxation.
CONCLUSIONS: The strikingly similar vascular function profiles of vessels incubated in the plasma of non-pregnant and pre-eclamptic women suggest that pre-eclampsia may be due, in part, to the lack of a necessary adaptation to pregnancy. Despite these similarities, however, there are differential effects of MMP inhibition, suggesting that MMPs play a greater role in the vasculature of women with pre-eclampsia compared with non-pregnant and normal pregnant women.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2004 |
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| Erschienen: |
2004 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:111 |
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| Enthalten in: |
BJOG : an international journal of obstetrics and gynaecology - 111(2004), 9 vom: 01. Sept., Seite 931-9 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Merchant, Shaila J [VerfasserIn] |
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| Anmerkungen: |
Date Completed 12.10.2004 Date Revised 03.06.2013 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM150064578 |
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| 041 | |a eng | ||
| 100 | 1 | |a Merchant, Shaila J |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The role of matrix metalloproteinases in vascular function |b implications for normal pregnancy and pre-eclampsia |
| 264 | 1 | |c 2004 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 12.10.2004 | ||
| 500 | |a Date Revised 03.06.2013 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVES: (1) To investigate the effects of plasma from non-pregnant, normal pregnant and pre-eclamptic women on myogenic tone and endothelium-dependent relaxation; (2) To investigate the role of matrix metalloproteinases (MMPs) in these parameters of vascular function | ||
| 520 | |a DESIGN: Analysis of the effects of plasma from non-pregnant, normal pregnant and pre-eclamptic women on myogenic tone and endothelium-dependent relaxation, and the effects of MMP inhibition on these parameters | ||
| 520 | |a SETTING: University of Alberta, Edmonton, Alberta, Canada | ||
| 520 | |a SAMPLES: Pooled plasma from non-pregnant (n= 9), normal pregnant (n= 6) and pre-eclamptic women (n= 12) | ||
| 520 | |a METHODS: Mesenteric arteries from virgin female C57BL/6J mice were exposed to pooled plasma from non-pregnant, normal pregnant and pre-eclamptic women in the absence and presence of GM6001 (5 microM; MMP inhibitor), and arterial function was assessed | ||
| 520 | |a MAIN OUTCOME MEASURES: Myogenic tone and endothelium-dependent relaxation | ||
| 520 | |a RESULTS: Plasma of women with pre-eclampsia, and interestingly, plasma of non-pregnant women, significantly enhanced myogenic tone at pressures > or =70 mmHg (P < 0.001) and blunted the maximum relaxation to methacholine (P < 0.001; P < 0.005) in isolated arteries. GM6001 altered response only in vessels exposed to the plasma of women with pre-eclampsia; however, contrary to our hypothesis, this further enhanced myogenic tone and further abrogated relaxation | ||
| 520 | |a CONCLUSIONS: The strikingly similar vascular function profiles of vessels incubated in the plasma of non-pregnant and pre-eclamptic women suggest that pre-eclampsia may be due, in part, to the lack of a necessary adaptation to pregnancy. Despite these similarities, however, there are differential effects of MMP inhibition, suggesting that MMPs play a greater role in the vasculature of women with pre-eclampsia compared with non-pregnant and normal pregnant women | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Dipeptides |2 NLM | |
| 650 | 7 | |a N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide |2 NLM | |
| 650 | 7 | |a Protease Inhibitors |2 NLM | |
| 650 | 7 | |a Matrix Metalloproteinases |2 NLM | |
| 650 | 7 | |a EC 3.4.24.- |2 NLM | |
| 700 | 1 | |a Davidge, Sandra T |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t BJOG : an international journal of obstetrics and gynaecology |d 2000 |g 111(2004), 9 vom: 01. Sept., Seite 931-9 |w (DE-627)NLM10574820X |x 1471-0528 |7 nnns |
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