Rapid vesicular translocation and insertion of TRP channels
The broadly expressed transient receptor potential (TRP) family of ion channels are permeant to cations, most resulting in increased intracellular calcium. However, their regulation and gating is not well understood. Here, we report that growth factor stimulation initiates the rapid translocation of the transient receptor potential ion channel, TRPC5, from vesicles held in reserve just under the plasma membrane. This process, which we term 'rapid vesicular insertion of TRP' (RiVIT), dramatically increases membrane-associated TRPC5 channels and functional TRPC5 current, resulting in tight spatial-temporal control of these Ca(2+)-permeant nonselective channels. Epidermal growth factor (EGF)-induced incorporation of functional TRP channels requires phosphatidylinositide 3-kinase (PI(3)K), the Rho GTPase Rac1 and phosphatidylinositol 4-phosphate 5-kinase (PIP(5)K alpha). The increase in TRPC5 availability affects neurite extension rates in cultured hippocampal neurons, and may be a general mechanism for initiating Ca(2+) influx and cell morphological changes in response to stimuli.
| Errataetall: |
CommentIn: Nat Cell Biol. 2004 Aug;6(8):690-2. - PMID 15303095 |
|---|---|
| Medienart: |
Artikel |
| Erscheinungsjahr: |
2004 |
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| Erschienen: |
2004 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
|---|---|
| Enthalten in: |
Nature cell biology - 6(2004), 8 vom: 23. Aug., Seite 709-20 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bezzerides, Vassilios J [VerfasserIn] |
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| Anmerkungen: |
Date Completed 27.08.2004 Date Revised 22.07.2023 published: Print-Electronic CommentIn: Nat Cell Biol. 2004 Aug;6(8):690-2. - PMID 15303095 Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| 500 | |a CommentIn: Nat Cell Biol. 2004 Aug;6(8):690-2. - PMID 15303095 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The broadly expressed transient receptor potential (TRP) family of ion channels are permeant to cations, most resulting in increased intracellular calcium. However, their regulation and gating is not well understood. Here, we report that growth factor stimulation initiates the rapid translocation of the transient receptor potential ion channel, TRPC5, from vesicles held in reserve just under the plasma membrane. This process, which we term 'rapid vesicular insertion of TRP' (RiVIT), dramatically increases membrane-associated TRPC5 channels and functional TRPC5 current, resulting in tight spatial-temporal control of these Ca(2+)-permeant nonselective channels. Epidermal growth factor (EGF)-induced incorporation of functional TRP channels requires phosphatidylinositide 3-kinase (PI(3)K), the Rho GTPase Rac1 and phosphatidylinositol 4-phosphate 5-kinase (PIP(5)K alpha). The increase in TRPC5 availability affects neurite extension rates in cultured hippocampal neurons, and may be a general mechanism for initiating Ca(2+) influx and cell morphological changes in response to stimuli | ||
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| 700 | 1 | |a Greka, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Clapham, David E |e verfasserin |4 aut | |
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